Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy

Junji Tada, Taiki Kono, Akira Suda, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa, Fumihiko Kannari

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.

Original languageEnglish
Pages (from-to)3023-3030
Number of pages8
JournalApplied Optics
Volume46
Issue number15
DOIs
Publication statusPublished - 2007 May 20

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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