Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy

Junji Tada, Taiki Kono, Akira Suda, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa, Fumihiko Kannari

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.

Original languageEnglish
Pages (from-to)3023-3030
Number of pages8
JournalApplied Optics
Volume46
Issue number15
DOIs
Publication statusPublished - 2007 May 20

Fingerprint

Fluorescence microscopy
Photons
microscopy
fluorescence
microstructure
learning
Microstructure
fibers
Fibers
photons
pulses
Fluorescence
excitation
proteins
Proteins
Pulse shaping
optimization
propagation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy. / Tada, Junji; Kono, Taiki; Suda, Akira; Mizuno, Hideaki; Miyawaki, Atsushi; Midorikawa, Katsumi; Kannari, Fumihiko.

In: Applied Optics, Vol. 46, No. 15, 20.05.2007, p. 3023-3030.

Research output: Contribution to journalArticle

Tada, Junji ; Kono, Taiki ; Suda, Akira ; Mizuno, Hideaki ; Miyawaki, Atsushi ; Midorikawa, Katsumi ; Kannari, Fumihiko. / Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy. In: Applied Optics. 2007 ; Vol. 46, No. 15. pp. 3023-3030.
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