Adenosine A2B receptor antagonist suppresses differentiation to regulatory T cells without suppressing activation of T cells

Hiroko Nakatsukasa, Mitsutoshi Tsukimoto, Hitoshi Harada, Shuji Kojima

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Extracellular adenosine activates P1 receptors (A1, A2A, A2B, A3) on cellular membranes. Here, we investigated the involvement of P1 receptor-mediated signaling in differentiation to regulatory T cells (Treg). Treg were induced in vitro by incubating isolated CD4+CD62L+ naïve murine T cells under Treg-skewing conditions. Antagonists of A1 and A2B receptors suppressed the expression of Foxp3, a specific marker of Treg, and the production of IL-10, suggesting the involvement of A1 and A2B receptors in differentiation to Treg. We also investigated the effect of these antagonists on T cell activation, which is essential for differentiation to Treg, and found that A1 antagonist, but not A2B antagonist, suppressed T cell activation. We conclude that A1 and A2B receptors are both involved in differentiation to Treg, but through different mechanisms. Since A2B antagonist blocked differentiation to Treg without suppressing T cell activation, it is possible that blockade of A2B receptor would facilitate tumor immunity.

Original languageEnglish
Pages (from-to)114-119
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume409
Issue number1
DOIs
Publication statusPublished - 2011 May 27
Externally publishedYes

Keywords

  • A1 receptor
  • A2B receptor
  • Adenosine
  • Foxp3
  • Regulatory T cell
  • T cell differentiation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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