Adenosine induces system A amino acid transport in cultured rat hepatocytes

Hiroaki Kiyokawa, Hiroyuki Fukui, Hiroyuki Mizuguchi, Akiharu Kubo, Norio Kono, Seiichiro Tarui, Hiroshi Wada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Adenosine caused a 2.5-fold increase in the sodium-dependent uptake of 2-aminoisobutyric acid in rat hepatocytes in primary culture following incubation for 3h. The range of stimulating concentrations of adenosine corresponded to that of cAMP formation. Adenosine increased the Vmax of the transport without altering the Km for 2-aminoisobutyric acid. These effects of adenosine were abolished by actinomycin D. N6-L-Phenylisopropyladenosine also caused twofold increase in the amino acid transport. These findings suggest that adenosine induces System A amino acid transport in a transcription-dependent manner, and that P,-purinergic receptors are mainly involved in this action of the nucleoside.

Original languageEnglish
Pages (from-to)9-11
Number of pages3
JournalJournal of Biochemistry
Volume110
Issue number1
Publication statusPublished - 1991 Jul
Externally publishedYes

Fingerprint

Amino Acid Transport Systems
Adenosine
Amino Acids
Amino acids
Rats
Hepatocytes
Acids
Transcription
Sodium
Phenylisopropyladenosine
Purinergic Receptors
Dependent
Dactinomycin
Nucleosides
Receptor
Fold
Rat
Range of data

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Kiyokawa, H., Fukui, H., Mizuguchi, H., Kubo, A., Kono, N., Tarui, S., & Wada, H. (1991). Adenosine induces system A amino acid transport in cultured rat hepatocytes. Journal of Biochemistry, 110(1), 9-11.

Adenosine induces system A amino acid transport in cultured rat hepatocytes. / Kiyokawa, Hiroaki; Fukui, Hiroyuki; Mizuguchi, Hiroyuki; Kubo, Akiharu; Kono, Norio; Tarui, Seiichiro; Wada, Hiroshi.

In: Journal of Biochemistry, Vol. 110, No. 1, 07.1991, p. 9-11.

Research output: Contribution to journalArticle

Kiyokawa, H, Fukui, H, Mizuguchi, H, Kubo, A, Kono, N, Tarui, S & Wada, H 1991, 'Adenosine induces system A amino acid transport in cultured rat hepatocytes', Journal of Biochemistry, vol. 110, no. 1, pp. 9-11.
Kiyokawa H, Fukui H, Mizuguchi H, Kubo A, Kono N, Tarui S et al. Adenosine induces system A amino acid transport in cultured rat hepatocytes. Journal of Biochemistry. 1991 Jul;110(1):9-11.
Kiyokawa, Hiroaki ; Fukui, Hiroyuki ; Mizuguchi, Hiroyuki ; Kubo, Akiharu ; Kono, Norio ; Tarui, Seiichiro ; Wada, Hiroshi. / Adenosine induces system A amino acid transport in cultured rat hepatocytes. In: Journal of Biochemistry. 1991 ; Vol. 110, No. 1. pp. 9-11.
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