Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus

Kazuhisa Moro, Akihiro Shiotani, Kazuhiko Watabe, Yasuo Takeda, Koichiro Saito, Yuko Mori, Kaoru Ogawa

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have previously shown that neuroprotective effects of an adenoviral glial cell line-derived neurotrophic factor (GDNF) gene transfer on the lesioned adult rat motoneurons in the nucleus ambiguus. In the present study, we examined neuroprotective effects of adenoviral gene transfer of brain-derived neurotrophic factor (BDNF) or/and GDNF to motoneurons in nucleus ambiguus using an adult rat vagal nerve avulsion model. The animals avulsed and inoculated with adenoviral vectors encoding BDNF (AxCAmBDNFME) or/and GDNF (AxCAhGDNF) showed immunolabeling for BDNF or/and GDNF in the nucleus ambiguus on the treated side, respectively, and expression of virus-induced BDNF or/and GDNF mRNA transcripts in the brainstem tissue that contained the nucleus ambiguus of the treated side. The treatment with AxCAhGDNF or AxCAmBDNFME significantly prevented the loss of vagal motoneurons in comparison to the control; the protective effect of AxCAmBDNFME was greater than that of AxCAhGDNF. The combined treatment with AxCAmBDNFME and AxCAhGDNF acted synergistically and significantly larger number of vagal motoneurons was preserved as compared to either AxCAmBDNFME treatment or AxCAhGDNF treatment. The treatment with AxCAmBDNFME or/and AxCAhGDNF after avulsion also suppressed the activity of nitric oxide synthase in lesioned motoneurons in the nucleus ambiguus. These results indicate that adenovirus-mediated BDNF and GDNF gene transfer may prevent the degeneration of motoneurons in humans after either vagal nerve injury or recurrent laryngeal nerve injury.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBrain Research
Volume1076
Issue number1
DOIs
Publication statusPublished - 2006 Mar 3

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Glial Cell Line-Derived Neurotrophic Factor
Medulla Oblongata
Brain-Derived Neurotrophic Factor
Motor Neurons
Genes
Neuroprotective Agents
Recurrent Laryngeal Nerve Injuries
Adenoviridae
Nitric Oxide Synthase
Brain Stem
Viruses
Messenger RNA
Wounds and Injuries

Keywords

  • Avulsion
  • BDNF
  • GDNF
  • Laryngeal paralysis
  • Motoneuron
  • Nucleus ambiguus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. / Moro, Kazuhisa; Shiotani, Akihiro; Watabe, Kazuhiko; Takeda, Yasuo; Saito, Koichiro; Mori, Yuko; Ogawa, Kaoru.

In: Brain Research, Vol. 1076, No. 1, 03.03.2006, p. 1-8.

Research output: Contribution to journalArticle

Moro, Kazuhisa ; Shiotani, Akihiro ; Watabe, Kazuhiko ; Takeda, Yasuo ; Saito, Koichiro ; Mori, Yuko ; Ogawa, Kaoru. / Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. In: Brain Research. 2006 ; Vol. 1076, No. 1. pp. 1-8.
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