Adenovirus-mediated gene transfer to the ocular surface epithelium

Kazuo Tsubota, Hiroko Inoue, Kiyoshi Ando, Masafumi Ono, Kenichi Yoshino, Ichiro Saito

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Abstract

Gene transfer to the ocular surface epithelium is of potential therapeutic value. It was determined whether a reporter gene can be introduced into the ocular surface epithelium in vitro (human cell lines), ex vivo (human tissues), and in vivo (rats) by treating with a recombinant, replication-deficient, adenovirus type 5. Human and conjunctival cell lines were cultured with various multiplicities of infection (MOI; 3.2 x 10-5-5 x 10-1) of adenovirus vector (Ad5:Adex1CAlacZ) containing the reporter gene lacZ (1-3-2.0 x 104 PFU ml-1). The ex vivo study used human corneal and conjunctival tissues obtained from an eye bank and during surgery. Non- specific upregulation of inflammatory cytokines of conjunctival epithelium infected by AdS was assayed and its suppression by steroids. For the in vivo study, Ad5 (5 x 105 PFU, 5-10 μl) was applied to the eyes of 8-12-week-old cotton rats, which were enucleated 24 and 48 hr later. The maximum lacZ expression in vitro was demonstrated in the corneal epithelial cell line at 7 days (1 x 10- MOI) and conjunctival epithelial cell line at 2 days (4 x 10- 4 MOI). Furthermore, lacZ was also expressed in the superficial corneal and conjunctival epithelium in the ex vivo study. IL-6, 11-8, and ICAM-1 expression from conjunctival epithelium by Ad5 was significantly inhibited by treatment with betamethasone (BM). For the in vivo study, only the conjunctival epithelium demonstrated β-Gal activity at 24 and 48 hr after application. These data indicate that adenovirus vector is capable of directly delivering gene to the corneal and conjunctival epithelium, suggesting a variety of possible gene therapy uses. The concomitant application of steroid eye drops may avoid inflammation.

Original languageEnglish
Pages (from-to)531-538
Number of pages8
JournalExperimental Eye Research
Volume67
Issue number5
DOIs
Publication statusPublished - 1998 Nov

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Keywords

  • β-galactosidase
  • Conjunctiva
  • Dry eye
  • Epithelium
  • Ocular surface

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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