Adenylate cyclase-coupled vasopressin receptor activates AQP2 promoter via a dual effect on CRE and API elements

Masato Yasui, Sergey M. Zelenin, Gianni Celsi, Anita Aperia

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

-Vasopressin plays an essential role for the regulation of water balance by activating the collecting duct-specific water channel, aquaporin-2 (AQP2). Here we present evidence that vasopressin may also act as a longterm, transcriptional regulator of AQP2. The studies were performed on LLC-PK1 cells, which normally express V2 receptor (V2R) and which were transfected with a fragment of the human AQP2 promoter. Activation of the adenylate cyclase-coupled V2R in LLC-PK1 cells induced phosphorylation of adenosine 3′,5′-cyclic monophosphate (cAMP) responsive element binding protein (CREB) and expression of c-Fos. Binding of these factors to the CRE and API site did, in combination, lead to AQP2 promoter activation. These results establish the role of vasopressin as a regulator of transcription and are the first example of how a message from a highly specific receptor is, via a dual effect of the cAMP signal on CREB and immediate early gene expression, transduced to the transcription of a final target protein with known biological effects.

Original languageEnglish
Pages (from-to)F451-F459
JournalAmerican Journal of Physiology
Volume272
Issue number4 PART 2
Publication statusPublished - 1997 Dec 1
Externally publishedYes

Keywords

  • Adenosine 3′,5′-cyclic monophosphate
  • Aquaporin-2
  • Gene regulation
  • Phosphorylation

ASJC Scopus subject areas

  • Physiology (medical)

Fingerprint Dive into the research topics of 'Adenylate cyclase-coupled vasopressin receptor activates AQP2 promoter via a dual effect on CRE and API elements'. Together they form a unique fingerprint.

Cite this