Adenylate cyclase-coupled vasopressin receptor activates aqp2 promoter via a dual effect on cre and api elements

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Abstract

-Vasopressin plavs an essential role for the regulation of water balance by activating the collecting duct-specific water channel, aquaporin-2 (AQP2). Here we present evidence that vasopressin may also act as a longterm, transcriptional regulator of AQP2. The studies were performed on LLC-PKi cells, which normally express V2 receptor (V2R) and which were transfected with a fragment of the human AQP2 promoter. Activation of the adenylate cyclase-coupled V2R in LLC-PK! cells induced phosphorylation of adenosine 3',5'-cyclic monophosphate (cAMP) responsive element binding protein (CREB) and expression of c-Fos. Binding of these factors to the CRE and API site did, in combination, lead to AQP2 promoter activation. These results establish the role of vasopressin as a regulator of transcription and are the first example of how a message from a highly specific receptor is, via a dual effect of the cAMP signal on CREB and immediate early gene expression, transduced to the transcription of a final target protein with known biological effects. gene regulation; phosphorylation; adenosine 3',5'-cyclic monophosphate; a.quaporin-2 I

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume41
Issue number4
Publication statusPublished - 1997
Externally publishedYes

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Aquaporin 2
Vasopressin Receptors
Adenylyl Cyclases
Vasopressins
Cyclic AMP
Carrier Proteins
Phosphorylation
Immediate-Early Genes
Gene Expression
Water
Genes
Proteins

ASJC Scopus subject areas

  • Physiology

Cite this

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title = "Adenylate cyclase-coupled vasopressin receptor activates aqp2 promoter via a dual effect on cre and api elements",
abstract = "-Vasopressin plavs an essential role for the regulation of water balance by activating the collecting duct-specific water channel, aquaporin-2 (AQP2). Here we present evidence that vasopressin may also act as a longterm, transcriptional regulator of AQP2. The studies were performed on LLC-PKi cells, which normally express V2 receptor (V2R) and which were transfected with a fragment of the human AQP2 promoter. Activation of the adenylate cyclase-coupled V2R in LLC-PK! cells induced phosphorylation of adenosine 3',5'-cyclic monophosphate (cAMP) responsive element binding protein (CREB) and expression of c-Fos. Binding of these factors to the CRE and API site did, in combination, lead to AQP2 promoter activation. These results establish the role of vasopressin as a regulator of transcription and are the first example of how a message from a highly specific receptor is, via a dual effect of the cAMP signal on CREB and immediate early gene expression, transduced to the transcription of a final target protein with known biological effects. gene regulation; phosphorylation; adenosine 3',5'-cyclic monophosphate; a.quaporin-2 I",
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T1 - Adenylate cyclase-coupled vasopressin receptor activates aqp2 promoter via a dual effect on cre and api elements

AU - Yasui, Masato

PY - 1997

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AB - -Vasopressin plavs an essential role for the regulation of water balance by activating the collecting duct-specific water channel, aquaporin-2 (AQP2). Here we present evidence that vasopressin may also act as a longterm, transcriptional regulator of AQP2. The studies were performed on LLC-PKi cells, which normally express V2 receptor (V2R) and which were transfected with a fragment of the human AQP2 promoter. Activation of the adenylate cyclase-coupled V2R in LLC-PK! cells induced phosphorylation of adenosine 3',5'-cyclic monophosphate (cAMP) responsive element binding protein (CREB) and expression of c-Fos. Binding of these factors to the CRE and API site did, in combination, lead to AQP2 promoter activation. These results establish the role of vasopressin as a regulator of transcription and are the first example of how a message from a highly specific receptor is, via a dual effect of the cAMP signal on CREB and immediate early gene expression, transduced to the transcription of a final target protein with known biological effects. gene regulation; phosphorylation; adenosine 3',5'-cyclic monophosphate; a.quaporin-2 I

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