Adhesion of lactobacillus to intestinal mucin

Keita Nishiyama, Takao Mukai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Adhesion to intestinal mucin is one of the main in vitro tests for the study of probiotic strains. Mucins immobilized on microtiter plates and glass slides are easy and excellent methods used for the quantitative analysis of Lactobacillus adhesion. However, to maintain the performance of the quantitative analysis, these methods must be chosen appropriately according to the nature and characteristics of the strain, such as tolerance to surfactant and the ability to self-aggregate. Here we describe two methodologies used to evaluate adhesion of Lactobacillus to mucin, in addition to the isolation and purification of mucins from porcine colonic tissues.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages159-166
Number of pages8
DOIs
Publication statusPublished - 2019 Jan 1
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1887
ISSN (Print)1064-3745

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Keywords

  • Adhesion
  • Aggregation
  • Diagnostic glass slide
  • Microtiter plate
  • Mucin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nishiyama, K., & Mukai, T. (2019). Adhesion of lactobacillus to intestinal mucin. In Methods in Molecular Biology (pp. 159-166). (Methods in Molecular Biology; Vol. 1887). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8907-2_14