Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins

Keita Nishiyama, Koichi Nakamata, Shintaro Ueno, Akari Terao, Ni Putu Desy Aryantini, I. Nengah Sujaya, Kenji Fukuda, Tadasu Urashima, Yuji Yamamoto, Takao Mukai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wildtype strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number2
DOIs
Publication statusPublished - 2015 Jan 1
Externally publishedYes

Fingerprint

Lactobacillus rhamnosus
Extracellular Matrix Proteins
Mucins
Mucus
Adhesion
Swine
Carrier Proteins
Milk
Fermented milk
Indonesia
Probiotics
Laminin
Fibronectins
Islands
Protein Binding
Assays
Collagen

Keywords

  • Bacterial adhesion
  • Extracellular matrix (ECM) proteins
  • Lactobacillus rhamnosus
  • Mucus-binding factor

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins. / Nishiyama, Keita; Nakamata, Koichi; Ueno, Shintaro; Terao, Akari; Aryantini, Ni Putu Desy; Sujaya, I. Nengah; Fukuda, Kenji; Urashima, Tadasu; Yamamoto, Yuji; Mukai, Takao.

In: Bioscience, Biotechnology and Biochemistry, Vol. 79, No. 2, 01.01.2015, p. 271-279.

Research output: Contribution to journalArticle

Nishiyama, K, Nakamata, K, Ueno, S, Terao, A, Aryantini, NPD, Sujaya, IN, Fukuda, K, Urashima, T, Yamamoto, Y & Mukai, T 2015, 'Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins', Bioscience, Biotechnology and Biochemistry, vol. 79, no. 2, pp. 271-279. https://doi.org/10.1080/09168451.2014.972325
Nishiyama, Keita ; Nakamata, Koichi ; Ueno, Shintaro ; Terao, Akari ; Aryantini, Ni Putu Desy ; Sujaya, I. Nengah ; Fukuda, Kenji ; Urashima, Tadasu ; Yamamoto, Yuji ; Mukai, Takao. / Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins. In: Bioscience, Biotechnology and Biochemistry. 2015 ; Vol. 79, No. 2. pp. 271-279.
@article{8d76522f9840407eb04355020db2f7a8,
title = "Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins",
abstract = "We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wildtype strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.",
keywords = "Bacterial adhesion, Extracellular matrix (ECM) proteins, Lactobacillus rhamnosus, Mucus-binding factor",
author = "Keita Nishiyama and Koichi Nakamata and Shintaro Ueno and Akari Terao and Aryantini, {Ni Putu Desy} and Sujaya, {I. Nengah} and Kenji Fukuda and Tadasu Urashima and Yuji Yamamoto and Takao Mukai",
year = "2015",
month = "1",
day = "1",
doi = "10.1080/09168451.2014.972325",
language = "English",
volume = "79",
pages = "271--279",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "2",

}

TY - JOUR

T1 - Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins

AU - Nishiyama, Keita

AU - Nakamata, Koichi

AU - Ueno, Shintaro

AU - Terao, Akari

AU - Aryantini, Ni Putu Desy

AU - Sujaya, I. Nengah

AU - Fukuda, Kenji

AU - Urashima, Tadasu

AU - Yamamoto, Yuji

AU - Mukai, Takao

PY - 2015/1/1

Y1 - 2015/1/1

N2 - We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wildtype strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.

AB - We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wildtype strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.

KW - Bacterial adhesion

KW - Extracellular matrix (ECM) proteins

KW - Lactobacillus rhamnosus

KW - Mucus-binding factor

UR - http://www.scopus.com/inward/record.url?scp=84952714296&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84952714296&partnerID=8YFLogxK

U2 - 10.1080/09168451.2014.972325

DO - 10.1080/09168451.2014.972325

M3 - Article

VL - 79

SP - 271

EP - 279

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 2

ER -