Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins

Keita Nishiyama, Koichi Nakamata, Shintaro Ueno, Akari Terao, Ni Putu Desy Aryantini, I. Nengah Sujaya, Kenji Fukuda, Tadasu Urashima, Yuji Yamamoto, Takao Mukai

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13 Citations (Scopus)

Abstract

We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wildtype strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • Bacterial adhesion
  • Extracellular matrix (ECM) proteins
  • Lactobacillus rhamnosus
  • Mucus-binding factor

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Nishiyama, K., Nakamata, K., Ueno, S., Terao, A., Aryantini, N. P. D., Sujaya, I. N., Fukuda, K., Urashima, T., Yamamoto, Y., & Mukai, T. (2015). Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins. Bioscience, Biotechnology and Biochemistry, 79(2), 271-279. https://doi.org/10.1080/09168451.2014.972325