Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

Daisuke Taura; Michio Noguchi; Masakatsu Sone; Kiminori Hosoda; Eisaku Mori; Yohei Okada; Kazutoshi Takahashi; Koichiro Homma; Naofumi Oyamada; Megumi Inuzuka; Takuhiro Sonoyama; Ken Ebihara; Naohisa Tamura; Hiroshi Itoh; Hirofumi Suemori; Norio Nakatsuji; Hideyuki Okano; Shinya Yamanaka; Kazuwa Nakao

doi:10.1016/j.febslet.2009.02.031

Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

Daisuke Taura, Michio Noguchi, Masakatsu Sone, Kiminori Hosoda, Eisaku Mori, Yohei Okada, Kazutoshi Takahashi, Koichiro Homma, Naofumi Oyamada, Megumi Inuzuka, Takuhiro Sonoyama, Ken Ebihara, Naohisa Tamura, Hiroshi Itoh, Hirofumi Suemori, Norio Nakatsuji, Hideyuki Okano, Shinya Yamanaka, Kazuwa Nakao

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125 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.

Original language	English
Pages (from-to)	1029-1033
Number of pages	5
Journal	FEBS Letters
Volume	583
Issue number	6
DOIs	https://doi.org/10.1016/j.febslet.2009.02.031
Publication status	Published - 2009 Mar 18

Keywords

Adipocyte
Adipogenesis
Differentiation
Stem cell

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Taura, D., Noguchi, M., Sone, M., Hosoda, K., Mori, E., Okada, Y., Takahashi, K., Homma, K., Oyamada, N., Inuzuka, M., Sonoyama, T., Ebihara, K., Tamura, N., Itoh, H., Suemori, H., Nakatsuji, N., Okano, H., Yamanaka, S., & Nakao, K. (2009). Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells. FEBS Letters, 583(6), 1029-1033. https://doi.org/10.1016/j.febslet.2009.02.031

Taura, D, Noguchi, M, Sone, M, Hosoda, K, Mori, E, Okada, Y, Takahashi, K, Homma, K, Oyamada, N, Inuzuka, M, Sonoyama, T, Ebihara, K, Tamura, N, Itoh, H, Suemori, H, Nakatsuji, N, Okano, H, Yamanaka, S & Nakao, K 2009, 'Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells', FEBS Letters, vol. 583, no. 6, pp. 1029-1033. https://doi.org/10.1016/j.febslet.2009.02.031

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title = "Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells",

abstract = "Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.",

keywords = "Adipocyte, Adipogenesis, Differentiation, Stem cell",

author = "Daisuke Taura and Michio Noguchi and Masakatsu Sone and Kiminori Hosoda and Eisaku Mori and Yohei Okada and Kazutoshi Takahashi and Koichiro Homma and Naofumi Oyamada and Megumi Inuzuka and Takuhiro Sonoyama and Ken Ebihara and Naohisa Tamura and Hiroshi Itoh and Hirofumi Suemori and Norio Nakatsuji and Hideyuki Okano and Shinya Yamanaka and Kazuwa Nakao",

note = "Funding Information: We thank Yoshie Fukuchi for her technical assistance. This work was supported by the project for realization of regenerative medicine of the Ministry of Education, Culture, Sports, Science and Technology, Japan.",

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doi = "10.1016/j.febslet.2009.02.031",

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T1 - Adipogenic differentiation of human induced pluripotent stem cells

T2 - Comparison with that of human embryonic stem cells

AU - Taura, Daisuke

AU - Noguchi, Michio

AU - Sone, Masakatsu

AU - Hosoda, Kiminori

AU - Mori, Eisaku

AU - Okada, Yohei

AU - Takahashi, Kazutoshi

AU - Homma, Koichiro

AU - Oyamada, Naofumi

AU - Inuzuka, Megumi

AU - Sonoyama, Takuhiro

AU - Ebihara, Ken

AU - Tamura, Naohisa

AU - Itoh, Hiroshi

AU - Suemori, Hirofumi

AU - Nakatsuji, Norio

AU - Okano, Hideyuki

AU - Yamanaka, Shinya

AU - Nakao, Kazuwa

N1 - Funding Information: We thank Yoshie Fukuchi for her technical assistance. This work was supported by the project for realization of regenerative medicine of the Ministry of Education, Culture, Sports, Science and Technology, Japan.

PY - 2009/3/18

Y1 - 2009/3/18

N2 - Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.

AB - Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.

KW - Adipocyte

KW - Adipogenesis

KW - Differentiation

KW - Stem cell

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Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

Abstract

Keywords

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