Administration of substance P during a primary immune response amplifies the secondary immune response via a long-lasting effect on CD8+ T lymphocytes

Yoshiki Ikeda, Hisato Takei, Chinami Matsumoto, Akihito Mase, Masahiro Yamamoto, Shuichi Takeda, Atsushi Ishige, Kenji Watanabe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Atopic dermatitis can be exacerbated or induced by scratching or psychological stress; both cause the release of substance P (SP) from sensory nerves. Therefore, SP may have an etiological role in mechanisms underlying AD. Here, we show that administration of SP during the primary immune response (PIR) imprinted long-lasting pro-inflammatory immunity, resulting in exacerbation of the secondary immune response (SIR) in the absence of further SP. Five days after sensitization with dinitrofluorobenzene (DNFB), challenge with DNFB together with SP ("SP-Group") resulted in an increased PIR (as evaluated by ear swelling and granulocyte infiltration) compared to DNFB only ("Control-Group"). On day 26, after inflammation completely subsided, a second challenge with DNFB only (without SP) caused an increased SIR in the "SP-Group" compared to controls. Pretreatment on day 5 with spantide, an SP receptor antagonist, prevented increased ear swelling in the "SP-Group" not only on day 5 (PIR) but also on day 26 (SIR). In contrast, spantide treatment on day 26 did not affect the SIR. Adoptive transfer experiments suggested that CD8+ T cells were involved in mediating enhanced SIR in animals pretreated on day 5 with SP. The present study offers a novel experimental approach to an uninvestigated facet of the pro-inflammatory effect of SP, i.e., exacerbation of inflammation via a long-term and indirect influence on CD8+ T lymphocytes.

Original languageEnglish
Pages (from-to)345-351
Number of pages7
JournalArchives of Dermatological Research
Volume299
Issue number7
DOIs
Publication statusPublished - 2007 Sep

Fingerprint

Substance P
T-Lymphocytes
Dinitrofluorobenzene
Ear
Neurokinin-1 Receptor Antagonists
Inflammation
Adoptive Transfer
Atopic Dermatitis
Psychological Stress
Granulocytes
Immunity
Control Groups

Keywords

  • Atopic dermatitis
  • CD8 T cells
  • Contact dermatitis
  • Long-term
  • Substance P

ASJC Scopus subject areas

  • Dermatology

Cite this

Administration of substance P during a primary immune response amplifies the secondary immune response via a long-lasting effect on CD8+ T lymphocytes. / Ikeda, Yoshiki; Takei, Hisato; Matsumoto, Chinami; Mase, Akihito; Yamamoto, Masahiro; Takeda, Shuichi; Ishige, Atsushi; Watanabe, Kenji.

In: Archives of Dermatological Research, Vol. 299, No. 7, 09.2007, p. 345-351.

Research output: Contribution to journalArticle

Ikeda, Yoshiki ; Takei, Hisato ; Matsumoto, Chinami ; Mase, Akihito ; Yamamoto, Masahiro ; Takeda, Shuichi ; Ishige, Atsushi ; Watanabe, Kenji. / Administration of substance P during a primary immune response amplifies the secondary immune response via a long-lasting effect on CD8+ T lymphocytes. In: Archives of Dermatological Research. 2007 ; Vol. 299, No. 7. pp. 345-351.
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