Adrenomedullin promotes proliferation and migration of cultured endothelial cells

Kazutoshi Miyashita, Hiroshi Itoh, Naoki Sawada, Yasutomo Fukunaga, Masakatsu Sone, Kenichi Yamahara, Takami Yurugi, Kazuwa Nakao

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Adrenomedullin (AM) Is a vasoactive hormone which exerts its action through cyclic adenosine monophosphate(cAMP) /cAMP-dependent protein kinase (PKA) cascade and intracellular Ca2+ mobilization. Recently, evidence has accumulated that AM plays a critical role in the regulation of vascular tone, remodeling and morphogenesis. And although numerous reports have examined the action of AM on cultured vascular cells, the results have not been consistent and have depended on the experimental conditions used. Accordingly, the purpose of this study was to clarify the effect of AM on the proliferation and migration of cultured endothelial cells. Our results revealed that AM promoted the growth and migration of endothelial cells (ECs). AM significantly promoted the proliferation of human umbilical vein endothelial cells (HUVECs) (56.0±8.7% over the controls at 10-9 mol/l) and this stimulative effect was inhibited by two AM antagonists, AM(22-52) and calcitonin gene-related peptide (CGRP) (8-37). The number of HUVECs migrated to the lower surface of the transwell apparatus was also increased dose-dependently in the AM group (30.4±4.2% over the controls at 10-7 mol/l), and this increase was suppressed by the two AM antagonists and by two PKA antagonists, adenosine 3′,5′-cyclic monophosphorothioate Rp-isomer and myristoylated protein kinase A inhibitor amide 14-22. The promoting action of AM on endothelial migration was also suppressed by LY294002, an inhibitor for phosphatidylinositol 3-kinase, but not by NG-nitro-L-arginine-methyl ester (L-NAME), an antagonist for nitric oxide synthase (NOS). These results indicate that AM promotes proliferation and migration of ECs via a cAMP/PKA dependent pathway and lend support to the idea that AM exerts beneficial effects on vascular regeneration and might be used as a novel therapeutic strategy for patients with vascular disease.

Original languageEnglish
JournalHypertension Research
Volume26
Issue numberSUPPL.
DOIs
Publication statusPublished - 2003 Feb
Externally publishedYes

Fingerprint

Adrenomedullin
Cultured Cells
Endothelial Cells
NG-Nitroarginine Methyl Ester
Human Umbilical Vein Endothelial Cells
Protein Kinases
Blood Vessels
Phosphatidylinositol 3-Kinase
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Adenosine Monophosphate
Cyclic AMP-Dependent Protein Kinases
Vascular Diseases
Morphogenesis
Nitric Oxide Synthase
Adenosine
Regeneration

Keywords

  • Adrenomedullin
  • Endothelial cells
  • Migration
  • Proliferation
  • Vascular regeneration

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Miyashita, K., Itoh, H., Sawada, N., Fukunaga, Y., Sone, M., Yamahara, K., ... Nakao, K. (2003). Adrenomedullin promotes proliferation and migration of cultured endothelial cells. Hypertension Research, 26(SUPPL.). https://doi.org/10.1291/hypres.26.S93

Adrenomedullin promotes proliferation and migration of cultured endothelial cells. / Miyashita, Kazutoshi; Itoh, Hiroshi; Sawada, Naoki; Fukunaga, Yasutomo; Sone, Masakatsu; Yamahara, Kenichi; Yurugi, Takami; Nakao, Kazuwa.

In: Hypertension Research, Vol. 26, No. SUPPL., 02.2003.

Research output: Contribution to journalArticle

Miyashita, K, Itoh, H, Sawada, N, Fukunaga, Y, Sone, M, Yamahara, K, Yurugi, T & Nakao, K 2003, 'Adrenomedullin promotes proliferation and migration of cultured endothelial cells', Hypertension Research, vol. 26, no. SUPPL.. https://doi.org/10.1291/hypres.26.S93
Miyashita, Kazutoshi ; Itoh, Hiroshi ; Sawada, Naoki ; Fukunaga, Yasutomo ; Sone, Masakatsu ; Yamahara, Kenichi ; Yurugi, Takami ; Nakao, Kazuwa. / Adrenomedullin promotes proliferation and migration of cultured endothelial cells. In: Hypertension Research. 2003 ; Vol. 26, No. SUPPL.
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abstract = "Adrenomedullin (AM) Is a vasoactive hormone which exerts its action through cyclic adenosine monophosphate(cAMP) /cAMP-dependent protein kinase (PKA) cascade and intracellular Ca2+ mobilization. Recently, evidence has accumulated that AM plays a critical role in the regulation of vascular tone, remodeling and morphogenesis. And although numerous reports have examined the action of AM on cultured vascular cells, the results have not been consistent and have depended on the experimental conditions used. Accordingly, the purpose of this study was to clarify the effect of AM on the proliferation and migration of cultured endothelial cells. Our results revealed that AM promoted the growth and migration of endothelial cells (ECs). AM significantly promoted the proliferation of human umbilical vein endothelial cells (HUVECs) (56.0±8.7{\%} over the controls at 10-9 mol/l) and this stimulative effect was inhibited by two AM antagonists, AM(22-52) and calcitonin gene-related peptide (CGRP) (8-37). The number of HUVECs migrated to the lower surface of the transwell apparatus was also increased dose-dependently in the AM group (30.4±4.2{\%} over the controls at 10-7 mol/l), and this increase was suppressed by the two AM antagonists and by two PKA antagonists, adenosine 3′,5′-cyclic monophosphorothioate Rp-isomer and myristoylated protein kinase A inhibitor amide 14-22. The promoting action of AM on endothelial migration was also suppressed by LY294002, an inhibitor for phosphatidylinositol 3-kinase, but not by NG-nitro-L-arginine-methyl ester (L-NAME), an antagonist for nitric oxide synthase (NOS). These results indicate that AM promotes proliferation and migration of ECs via a cAMP/PKA dependent pathway and lend support to the idea that AM exerts beneficial effects on vascular regeneration and might be used as a novel therapeutic strategy for patients with vascular disease.",
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