Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces

Akihiko Kikuchi, Tetsuya Endo, Jun Kobayashi, Hideko Kanazawa, Kiyotaka Sakai, Teruo Okano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In the present research, we newly developed thermoresponsive nanobio interfaces for possible applications in affinity regulations. We introduced thermoresponsive polymers having metal chelating segments (iminodiacetic acid moiety on the side chains) onto animated silica beads surfaces. Utilizing thermoresponsive expanding-contracting changes of poly(N-isopropylacrylamide) on the bead surfaces, we succeeded in on-off regulation of specific affinity interactions between immobilized metal ligands and lysozyme having one histidine residue. The obtained results suggest that affinity modulation can be achieved by utilizing thermally regulated polymer chain hydration changes without any use of competitive agents.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages4719-4720
Number of pages2
Volume54
Edition2
Publication statusPublished - 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Fingerprint

Ligands
Proteins
Polymers
Chelation
Metals
Hydration
Enzymes
Silica
Modulation
Acids

Keywords

  • Chromatography
  • Immobilized metal affinity ligands
  • Poly(N-isopropylacrylamide)
  • Protein separation
  • Ultrathin polymer interfaces

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kikuchi, A., Endo, T., Kobayashi, J., Kanazawa, H., Sakai, K., & Okano, T. (2005). Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces. In Polymer Preprints, Japan (2 ed., Vol. 54, pp. 4719-4720)

Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces. / Kikuchi, Akihiko; Endo, Tetsuya; Kobayashi, Jun; Kanazawa, Hideko; Sakai, Kiyotaka; Okano, Teruo.

Polymer Preprints, Japan. Vol. 54 2. ed. 2005. p. 4719-4720.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kikuchi, A, Endo, T, Kobayashi, J, Kanazawa, H, Sakai, K & Okano, T 2005, Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces. in Polymer Preprints, Japan. 2 edn, vol. 54, pp. 4719-4720, 54th SPSJ Symposium on Macromolecules, Yamagata, Japan, 05/9/20.
Kikuchi A, Endo T, Kobayashi J, Kanazawa H, Sakai K, Okano T. Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces. In Polymer Preprints, Japan. 2 ed. Vol. 54. 2005. p. 4719-4720
Kikuchi, Akihiko ; Endo, Tetsuya ; Kobayashi, Jun ; Kanazawa, Hideko ; Sakai, Kiyotaka ; Okano, Teruo. / Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces. Polymer Preprints, Japan. Vol. 54 2. ed. 2005. pp. 4719-4720
@inproceedings{ccc117e1ceb84bcfb66b58633edf8f34,
title = "Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces",
abstract = "In the present research, we newly developed thermoresponsive nanobio interfaces for possible applications in affinity regulations. We introduced thermoresponsive polymers having metal chelating segments (iminodiacetic acid moiety on the side chains) onto animated silica beads surfaces. Utilizing thermoresponsive expanding-contracting changes of poly(N-isopropylacrylamide) on the bead surfaces, we succeeded in on-off regulation of specific affinity interactions between immobilized metal ligands and lysozyme having one histidine residue. The obtained results suggest that affinity modulation can be achieved by utilizing thermally regulated polymer chain hydration changes without any use of competitive agents.",
keywords = "Chromatography, Immobilized metal affinity ligands, Poly(N-isopropylacrylamide), Protein separation, Ultrathin polymer interfaces",
author = "Akihiko Kikuchi and Tetsuya Endo and Jun Kobayashi and Hideko Kanazawa and Kiyotaka Sakai and Teruo Okano",
year = "2005",
language = "English",
volume = "54",
pages = "4719--4720",
booktitle = "Polymer Preprints, Japan",
edition = "2",

}

TY - GEN

T1 - Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces

AU - Kikuchi, Akihiko

AU - Endo, Tetsuya

AU - Kobayashi, Jun

AU - Kanazawa, Hideko

AU - Sakai, Kiyotaka

AU - Okano, Teruo

PY - 2005

Y1 - 2005

N2 - In the present research, we newly developed thermoresponsive nanobio interfaces for possible applications in affinity regulations. We introduced thermoresponsive polymers having metal chelating segments (iminodiacetic acid moiety on the side chains) onto animated silica beads surfaces. Utilizing thermoresponsive expanding-contracting changes of poly(N-isopropylacrylamide) on the bead surfaces, we succeeded in on-off regulation of specific affinity interactions between immobilized metal ligands and lysozyme having one histidine residue. The obtained results suggest that affinity modulation can be achieved by utilizing thermally regulated polymer chain hydration changes without any use of competitive agents.

AB - In the present research, we newly developed thermoresponsive nanobio interfaces for possible applications in affinity regulations. We introduced thermoresponsive polymers having metal chelating segments (iminodiacetic acid moiety on the side chains) onto animated silica beads surfaces. Utilizing thermoresponsive expanding-contracting changes of poly(N-isopropylacrylamide) on the bead surfaces, we succeeded in on-off regulation of specific affinity interactions between immobilized metal ligands and lysozyme having one histidine residue. The obtained results suggest that affinity modulation can be achieved by utilizing thermally regulated polymer chain hydration changes without any use of competitive agents.

KW - Chromatography

KW - Immobilized metal affinity ligands

KW - Poly(N-isopropylacrylamide)

KW - Protein separation

KW - Ultrathin polymer interfaces

UR - http://www.scopus.com/inward/record.url?scp=33645580175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645580175&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:33645580175

VL - 54

SP - 4719

EP - 4720

BT - Polymer Preprints, Japan

ER -