Affinity control of immobilized metal ligands with proteins using thermoresponsive nanogel-like interfaces

Akihiko Kikuchi, Tetsuya Endo, Jun Kobayashi, Hideko Kanazawa, Kiyotaka Sakai, Teruo Okano

Research output: Contribution to conferencePaperpeer-review

Abstract

In the present research, we newly developed thermoresponsive nanobio interfaces for possible applications in affinity regulations. We introduced thermoresponsive polymers having metal chelating segments (iminodiacetic acid moiety on the side chains) onto animated silica beads surfaces. Utilizing thermoresponsive expanding-contracting changes of poly(N-isopropylacrylamide) on the bead surfaces, we succeeded in on-off regulation of specific affinity interactions between immobilized metal ligands and lysozyme having one histidine residue. The obtained results suggest that affinity modulation can be achieved by utilizing thermally regulated polymer chain hydration changes without any use of competitive agents.

Original languageEnglish
Pages4719-4720
Number of pages2
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Keywords

  • Chromatography
  • Immobilized metal affinity ligands
  • Poly(N-isopropylacrylamide)
  • Protein separation
  • Ultrathin polymer interfaces

ASJC Scopus subject areas

  • Engineering(all)

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