Affinity discrimination of DNA on SPR sensor using oligolysine-immobilized particle

Kaori Umehara, Kazunobu Toshima, Haruma Kawaguchi

Research output: Contribution to conferencePaper

Abstract

We have made an attempt to detect bulge DNA by surface plasmon resonance (SPR) biosensor. Peptide nucleic acid (PNA) was conjugated onto polymeric microsphere, which had been prepared by soap-free emulsion polymerization. SPR results indicated that PNA-conjugated microsphere can detect bulge DNA, and amplify SPR signal. Furthermore, we succeeded in magnifying SPR response more than ten times maintaining high discrimination ability by oligolysine-PNA immobilized microsphere.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period06/5/2406/5/26

Keywords

  • Affinity particle
  • Bulge DNA
  • DNA discrimination
  • Oligolysine
  • SPR (surface plasmon resonance) sensor

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Umehara, K., Toshima, K., & Kawaguchi, H. (2006). Affinity discrimination of DNA on SPR sensor using oligolysine-immobilized particle. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.