AFM imagings of ferritin molecules bound to LB films of poly-1-benzyl-L-histidine

Satomi Ohnishi, Masahiko Hara, Taiji Furuno, Wolfgang Knoll, Hiroyuki Sasabe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

9 Citations (Scopus)

Abstract

A water-soluble protein, ferritin, on a silicon surface has been imaged in pure water at room temperature with the atomic force microscope (AFM). The sample were prepared by binding ferritin molecules electrostatically to a charged polypeptide layer of poly-1-benzyl-L-histidine (PBLH). The hexagonal arrangement of ferritin molecules was imaged with high reproducibility, since the force between tip and the sample surface could be kept sufficiently lower than 10-10 N. The applied force can be stabilized and weakened mainly due to a 'self-screening effect' of the surface charges of the ferritin-PBLH layer. We demonstrate that the electrostatic-binding sample preparation is one of the suitable methods for soft biological specimens to achieve the nondestructive low-force AFM imagings.

Original languageEnglish
Title of host publicationMaterials Research Society Symposium Proceedings
PublisherPubl by Materials Research Society
Pages145-150
Number of pages6
ISBN (Print)1558991905
Publication statusPublished - 1993 Jan 1
EventSymposium on Atomic-scale Imaging of Surfaces and Interfaces -
Duration: 1992 Nov 301992 Dec 2

Publication series

NameMaterials Research Society Symposium Proceedings
Volume295
ISSN (Print)0272-9172

Other

OtherSymposium on Atomic-scale Imaging of Surfaces and Interfaces
Period92/11/3092/12/2

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ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Ohnishi, S., Hara, M., Furuno, T., Knoll, W., & Sasabe, H. (1993). AFM imagings of ferritin molecules bound to LB films of poly-1-benzyl-L-histidine. In Materials Research Society Symposium Proceedings (pp. 145-150). (Materials Research Society Symposium Proceedings; Vol. 295). Publ by Materials Research Society.