Age-associated alteration of gene expression patterns in mouse oocytes

Toshio Hamatani, Geppino Falco, Mark G. Carter, Hidenori Akutsu, Carole A. Stagg, Alexei A. Sharov, Dawood B. Dudekula, Vincent VanBuren, Minoru Ko

Research output: Contribution to journalArticle

318 Citations (Scopus)

Abstract

Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5- to 6-week-old mice with those collected from 42- to 45-week-old mice using the NIA 22K 60-mer oligo microarray. Among approximately 11 000 genes whose transcripts were detected in oocytes, about 5% (530) showed statistically significant expression changes, excluding the possibility of global decline in transcript abundance. Consistent with the generally accepted view of aging, the differentially expressed genes included ones involved in mitochondrial function and oxidative stress. However, the expression of other genes involved in chromatin structure, DNA methylation, genome stability and RNA helicases was also altered, suggesting the existence of additional mechanisms for aging. Among the transcripts decreased with aging, we identified and characterized a group of new oocyte-specific genes, members of the human NACHT, leucine-rich repeat and PYD-containing (NALP) gene family. These results have implications for aging research as well as for clinical ooplasmic donation to rejuvenate aging oocytes.

Original languageEnglish
Pages (from-to)2263-2278
Number of pages16
JournalHuman Molecular Genetics
Volume13
Issue number19
DOIs
Publication statusPublished - 2004 Oct 1
Externally publishedYes

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Oocytes
Gene Expression
Genes
RNA Helicases
Genomic Instability
DNA Methylation
Metaphase
Leucine
Mental Competency
Infertility
Chromatin
Oxidative Stress
Mothers
Research

ASJC Scopus subject areas

  • Genetics

Cite this

Age-associated alteration of gene expression patterns in mouse oocytes. / Hamatani, Toshio; Falco, Geppino; Carter, Mark G.; Akutsu, Hidenori; Stagg, Carole A.; Sharov, Alexei A.; Dudekula, Dawood B.; VanBuren, Vincent; Ko, Minoru.

In: Human Molecular Genetics, Vol. 13, No. 19, 01.10.2004, p. 2263-2278.

Research output: Contribution to journalArticle

Hamatani, T, Falco, G, Carter, MG, Akutsu, H, Stagg, CA, Sharov, AA, Dudekula, DB, VanBuren, V & Ko, M 2004, 'Age-associated alteration of gene expression patterns in mouse oocytes', Human Molecular Genetics, vol. 13, no. 19, pp. 2263-2278. https://doi.org/10.1093/hmg/ddh241
Hamatani T, Falco G, Carter MG, Akutsu H, Stagg CA, Sharov AA et al. Age-associated alteration of gene expression patterns in mouse oocytes. Human Molecular Genetics. 2004 Oct 1;13(19):2263-2278. https://doi.org/10.1093/hmg/ddh241
Hamatani, Toshio ; Falco, Geppino ; Carter, Mark G. ; Akutsu, Hidenori ; Stagg, Carole A. ; Sharov, Alexei A. ; Dudekula, Dawood B. ; VanBuren, Vincent ; Ko, Minoru. / Age-associated alteration of gene expression patterns in mouse oocytes. In: Human Molecular Genetics. 2004 ; Vol. 13, No. 19. pp. 2263-2278.
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