Age-related changes of a-tocopherol transfer protein expression in rat liver

Han Suk Kim, Hiroyuki Arai, Makoto Arita, Yuji Sato, Tohru Ogihara, Hiroshi Tamai, Keizo Inoue, Makoto Mino

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Developmental changes in expression of α-tocopherol transfer protein (α-TTP) after birth were investigated using rats with respect to plasma changes of tocopherols. The expression of α-TTP in the neonatal rat liver, which was very low immediately after birth, increased steadily during the two weeks of life before weanling and reached the adult level at four weeks. During the suckling period, the plasma ratio of α-tocopherol to γ-tocopherol linearly increased, because plasma α-tocopherol which was low immediately after birth, increased rapidly during the period, while γ-tocopherol remained unchanged. The increase in the ratio seemed to correlate with the developmental expression of α-TTP in the liver during this period. The ratio also reached the adult level after four weeks. The expression of α-TTP was investigated using primary cultured rat hepatocytes. The expression of α-TTP was found to be extremely low after 20h of culture. The decrease in α-TTP expression was exacerbated by adding epidermal growth factor to the culture medium and was inhibited by adding dexamethasone. These observations suggest that expression of α-TTP may be affected by the state of hepatic differentiation.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalJournal of Nutritional Science and Vitaminology
Volume42
Issue number1
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Tocopherols
tocopherols
protein synthesis
liver
Liver
rats
Proteins
Parturition
epidermal growth factor
dexamethasone
weanlings
suckling
Epidermal Growth Factor
Dexamethasone
hepatocytes
Culture Media
thymidine 5'-triphosphate
Hepatocytes
neonates
culture media

Keywords

  • α-tocopherol
  • α-tocopherol transfer protein
  • γ-tocopherol
  • Aging
  • Cell differentiation
  • Discrimination of tocopherols
  • Hepatocyte
  • Primary culture

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Kim, H. S., Arai, H., Arita, M., Sato, Y., Ogihara, T., Tamai, H., ... Mino, M. (1996). Age-related changes of a-tocopherol transfer protein expression in rat liver. Journal of Nutritional Science and Vitaminology, 42(1), 11-18.

Age-related changes of a-tocopherol transfer protein expression in rat liver. / Kim, Han Suk; Arai, Hiroyuki; Arita, Makoto; Sato, Yuji; Ogihara, Tohru; Tamai, Hiroshi; Inoue, Keizo; Mino, Makoto.

In: Journal of Nutritional Science and Vitaminology, Vol. 42, No. 1, 1996, p. 11-18.

Research output: Contribution to journalArticle

Kim, HS, Arai, H, Arita, M, Sato, Y, Ogihara, T, Tamai, H, Inoue, K & Mino, M 1996, 'Age-related changes of a-tocopherol transfer protein expression in rat liver', Journal of Nutritional Science and Vitaminology, vol. 42, no. 1, pp. 11-18.
Kim, Han Suk ; Arai, Hiroyuki ; Arita, Makoto ; Sato, Yuji ; Ogihara, Tohru ; Tamai, Hiroshi ; Inoue, Keizo ; Mino, Makoto. / Age-related changes of a-tocopherol transfer protein expression in rat liver. In: Journal of Nutritional Science and Vitaminology. 1996 ; Vol. 42, No. 1. pp. 11-18.
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