Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor receptor

Role of cytoplasmic tail phosphorylation in each process

Isao Ishii, Erika Saito, Takashi Izumi, Michio Ui, Takao Shimizu

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor (PAF) receptor were characterized in transfected Chinese hamster ovary cells. Exposure of the cells to PAF led to rapid sequestration of the receptors into the intracellular compartment and desensitization of the response to PAF. The sequestration was inhibited by pretreatments that perturbed the clathrin-mediated pathway. Subsequent removal of PAF by washing with receptor antagonists led to rapid recycling of the sequestered receptors to the cell surface accompanied by resensitization to PAF. To evaluate the potential role of phosphorylation in the receptor cytoplasmic tail during these processes, mutant receptors in which the tails were truncated or substituted, so as to lack serine/threonine residues, were created. PAF phosphorylated the wildtype receptor rapidly and strongly, but the mutants did not. The maximal extent of sequestration of each mutant was lower than that of the wild-type, and one of the substituted mutants showed no sequestration. Furthermore, the sequestration-defective mutant showed evidence of desensitization after agonist stimulation but not resensitization after agonist removal. Thus, agonist-induced phosphorylation of the cytoplasmic tail facilitates but is not essential for receptor sequestration, and sequestration/recycling appears important in receptor resensitization.

Original languageEnglish
Pages (from-to)9878-9885
Number of pages8
JournalJournal of Biological Chemistry
Volume273
Issue number16
DOIs
Publication statusPublished - 1998 Apr 17
Externally publishedYes

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Phosphorylation
Platelet Activating Factor
Recycling
Tail
Clathrin
Cell Surface Receptors
Threonine
Cytoplasmic and Nuclear Receptors
Cricetulus
Washing
Serine
Ovary
Cells
platelet activating factor receptor

ASJC Scopus subject areas

  • Biochemistry

Cite this

Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor receptor : Role of cytoplasmic tail phosphorylation in each process. / Ishii, Isao; Saito, Erika; Izumi, Takashi; Ui, Michio; Shimizu, Takao.

In: Journal of Biological Chemistry, Vol. 273, No. 16, 17.04.1998, p. 9878-9885.

Research output: Contribution to journalArticle

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