Airway M Cells arise in the lower airway due to RANKL signaling and reside in the bronchiolar epithelium associated with iBALT in murine models of respiratory disease

Shunsuke Kimura, Mami Mutoh, Meri Hisamoto, Hikaru Saito, Shun Takahashi, Takanori Asakura, Makoto Ishii, Yutaka Nakamura, Junichiro Iida, Kouji Hase, Toshihiko Iwanaga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Microfold (M) cells residing in the follicle-associated epithelium of mucosa-associated lymphoid tissues are specialized for sampling luminal antigens to initiate mucosal immune responses. In the past decade, glycoprotein 2 (GP2) and Tnfaip2 were identified as reliable markers for M cells in the Peyer's patches of the intestine. Furthermore, RANKL-RANK signaling, as well as the canonical and non-canonical NFκB pathways downstream, is essential for M-cell differentiation from the intestinal stem cells. However, the molecular characterization and differentiation mechanisms of M cells in the lower respiratory tract, where organized lymphoid tissues exist rarely, remain to be fully elucidated. Therefore, this study aimed to explore M cells in the lower respiratory tract in terms of their specific molecular markers, differentiation mechanism, and functions. Immunofluorescence analysis revealed a small number of M cells expressing GP2, Tnfaip2, and RANK is present in the lower respiratory tract of healthy mice. The intraperitoneal administration of RANKL in mice effectively induced M cells, which have a high capacity to take up luminal substrates, in the lower respiratory epithelium. The airway M cells associated with lymphoid follicles were frequently detected in the pathologically induced bronchus-associated lymphoid tissue (iBALT) in the murine models of autoimmune disease as well as pulmonary emphysema. These findings demonstrate that RANKL is a common inducer of M cells in the airway and digestive tracts and that M cells are associated with the respiratory disease. We also established a two-dimensional culture method for airway M cells from the tracheal epithelium in the presence of RANKL successfully. This model may be useful for functional studies of M cells in the sampling of antigens at airway mucosal surfaces.

Original languageEnglish
Article number1323
JournalFrontiers in Immunology
Volume10
Issue numberJUN
DOIs
Publication statusPublished - 2019 Jan 1

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Lymphoid Tissue
Bronchi
Epithelium
Respiratory System
Glycoproteins
Antigens
Respiratory Mucosa
Peyer's Patches
Mucosal Immunity
Pulmonary Emphysema
Differentiation Antigens
Helper-Inducer T-Lymphocytes
Autoimmune Diseases
Intestines
Fluorescent Antibody Technique
Gastrointestinal Tract
Cell Differentiation
Mucous Membrane
Stem Cells
Cell Count

Keywords

  • GP2
  • IBALT
  • Lower airway
  • M cells
  • Microfold cells
  • RANKL

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Airway M Cells arise in the lower airway due to RANKL signaling and reside in the bronchiolar epithelium associated with iBALT in murine models of respiratory disease. / Kimura, Shunsuke; Mutoh, Mami; Hisamoto, Meri; Saito, Hikaru; Takahashi, Shun; Asakura, Takanori; Ishii, Makoto; Nakamura, Yutaka; Iida, Junichiro; Hase, Kouji; Iwanaga, Toshihiko.

In: Frontiers in Immunology, Vol. 10, No. JUN, 1323, 01.01.2019.

Research output: Contribution to journalArticle

Kimura, Shunsuke ; Mutoh, Mami ; Hisamoto, Meri ; Saito, Hikaru ; Takahashi, Shun ; Asakura, Takanori ; Ishii, Makoto ; Nakamura, Yutaka ; Iida, Junichiro ; Hase, Kouji ; Iwanaga, Toshihiko. / Airway M Cells arise in the lower airway due to RANKL signaling and reside in the bronchiolar epithelium associated with iBALT in murine models of respiratory disease. In: Frontiers in Immunology. 2019 ; Vol. 10, No. JUN.
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