Airway reconstruction using decellularized tracheal allografts in a porcine model

Michinobu Ohno; Yasushi Fuchimoto; Huai Che Hsu; Masataka Higuchi; Makoto Komura; Tetsuji Yamaoka; Akihiro Umezawa; Shin Enosawa; Tatsuo Kuroda

doi:10.1007/s00383-017-4138-8

Airway reconstruction using decellularized tracheal allografts in a porcine model

Michinobu Ohno, Yasushi Fuchimoto, Huai Che Hsu, Masataka Higuchi, Makoto Komura, Tetsuji Yamaoka, Akihiro Umezawa, Shin Enosawa, Tatsuo Kuroda

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Purpose: Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. Methods: Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies. Results: No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts. Conclusion: Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.

Original language	English
Pages (from-to)	1065-1071
Number of pages	7
Journal	Pediatric surgery international
Volume	33
Issue number	10
DOIs	https://doi.org/10.1007/s00383-017-4138-8
Publication status	Published - 2017 Oct 1

Keywords

Decellularized trachea
Porcine model
Regeneration
Tissue-engineering
Tracheal reconstruction

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Surgery

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10.1007/s00383-017-4138-8

Cite this

@article{104011c9d8b6466a8b8b62a0629e2a1d,

title = "Airway reconstruction using decellularized tracheal allografts in a porcine model",

abstract = "Purpose: Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. Methods: Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies. Results: No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts. Conclusion: Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.",

keywords = "Decellularized trachea, Porcine model, Regeneration, Tissue-engineering, Tracheal reconstruction",

author = "Michinobu Ohno and Yasushi Fuchimoto and Hsu, {Huai Che} and Masataka Higuchi and Makoto Komura and Tetsuji Yamaoka and Akihiro Umezawa and Shin Enosawa and Tatsuo Kuroda",

note = "Funding Information: Funding This work was supported by JSPS KAKENHI (grant numbers JP16K20348 and JP16K11359) and a Grant-in-Aid from the National Center for Child Health and Development (grant number 27-8). Funding Information: Acknowledgements We thank Mr. Minoru Ichinose and Ms. Hiroko Komura for their excellent technical assistance and Mr. Jyunpei Yamamoto for assistance with the high-pressure treatment of tissues. This work was supported by JSPS KAKENHI (Grant Numbers JP16K20348 and JP16K11359) and a Grant-in-Aid from the National Center for Child Health and Development (Grant Number 27-8). Publisher Copyright: {\textcopyright} 2017, Springer-Verlag GmbH Germany.",

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month = oct,

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doi = "10.1007/s00383-017-4138-8",

language = "English",

volume = "33",

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TY - JOUR

T1 - Airway reconstruction using decellularized tracheal allografts in a porcine model

AU - Ohno, Michinobu

AU - Fuchimoto, Yasushi

AU - Hsu, Huai Che

AU - Higuchi, Masataka

AU - Komura, Makoto

AU - Yamaoka, Tetsuji

AU - Umezawa, Akihiro

AU - Enosawa, Shin

AU - Kuroda, Tatsuo

N1 - Funding Information: Funding This work was supported by JSPS KAKENHI (grant numbers JP16K20348 and JP16K11359) and a Grant-in-Aid from the National Center for Child Health and Development (grant number 27-8). Funding Information: Acknowledgements We thank Mr. Minoru Ichinose and Ms. Hiroko Komura for their excellent technical assistance and Mr. Jyunpei Yamamoto for assistance with the high-pressure treatment of tissues. This work was supported by JSPS KAKENHI (Grant Numbers JP16K20348 and JP16K11359) and a Grant-in-Aid from the National Center for Child Health and Development (Grant Number 27-8). Publisher Copyright: © 2017, Springer-Verlag GmbH Germany.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Purpose: Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. Methods: Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies. Results: No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts. Conclusion: Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.

AB - Purpose: Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. Methods: Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies. Results: No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts. Conclusion: Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.

KW - Decellularized trachea

KW - Porcine model

KW - Regeneration

KW - Tissue-engineering

KW - Tracheal reconstruction

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SN - 0179-0358

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JO - Pediatric Surgery International

JF - Pediatric Surgery International

IS - 10

ER -

Airway reconstruction using decellularized tracheal allografts in a porcine model

Abstract

Keywords

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