Albumin uptake into endothelial cells in separated flow

Susumu Kudo, Ryuhei Yamaguchi, Masashi Sato, Kotaro Oka, Kazuo Tanishita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purpose of this study is to reveal the albumin uptake intoendothelial cells in the separated flow area. After 24 hr of exposure toflow induced in a back step flow channel, the endothelial cells wereincubated in 371C for 60 minutes in PBS containingtetramethylrhodamine isothiocyanate conjugated albumin (TRITCalbumin). Thereafter, the cell morphology and the albumin uptakewere observed by a confocal laser scanning microscope (CLSM). In alow shear stress area (stagnant and reattachment areas), the cellsaligned randomly. In a high shear stress area (reversal and fullydeveloped areas), the cells were elongated and aligned to flowdirection. In low-shear-stress and high-shear-stress gradient areas(reattachment areas), the amount of albumin uptake into the cells wasthe largest in all areas. These data indicate that shear stress and shearstress gradient affect the endothelial cell morphology and the albuminuptake into endothelial cells.

Original languageEnglish
Title of host publicationAdvances in Bioengineering
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages83-84
Number of pages2
ISBN (Electronic)9780791819340
DOIs
Publication statusPublished - 2000
EventASME 2000 International Mechanical Engineering Congress and Exposition, IMECE 2000 - Orlando, United States
Duration: 2000 Nov 52000 Nov 10

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume2000-D

Conference

ConferenceASME 2000 International Mechanical Engineering Congress and Exposition, IMECE 2000
Country/TerritoryUnited States
CityOrlando
Period00/11/500/11/10

ASJC Scopus subject areas

  • Mechanical Engineering

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