Alcohol dehydrogenase activities in the human gastric mucosa: Effects of Helicobacter pylori infection, sex, age, and the part of the stomach

Michinaga Matsumoto, Hirokazu Yokoyama, Haruko Shiraishi, Hidekazu Suzuki, Shinzo Kato, Souichiro Miura, Hiromasa Ishii

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Human gastric mucosa contains three alcohol dehydrogenase (ADH) isozymes (classes I, III, and IV). Various factors such as Helicobacter pylori infection, sex, age, and the part of the stomach involved have been suggested to affect alcohol dehydrogenase activities, although these views are controversial. In this study, these unsettled issues were reexamined. Methods: Activities of class I and IV ADHs were evaluated in the cytosolic fraction of human gastric mucosa samples by reduction of their preferred substrates, namely acetaldehyde and m-nitrobenzaldehyde, and activities of class III were evaluated by oxidation of its preferred substrate, formaldehyde. Then, effects of Helicobacter pylori infection, sex, age, and the part of the stomach involved were examined. Results: Class I, III, and IV ADH activities were 17.5 ± 8.4, 4.2 ± 2.5, and 8.9 ± 3.9 nmol of nicotinamide adenine dinucleotide oxidation per minute per milligram of protein, respectively, for the entire population. Helicobacter pylori infection significantly reduced class I and IV ADH activities but did not affect activity of class III. In the samples without Helicobacter pylori infection and severe gastritis, sex did not affect class I, III, or IV ADH activities. In the same series, class IV ADH activity significantly decreased with age (p = 0.006), whereas no correlation was found between age and ADH activity of class I and III ADHs. The level of class IV ADH activity was significantly higher in the upper body than in the lower regions, whereas no such heterogeneity was observed in class I and III ADH. Conclusions: Various factors affect human gastric ADH activities, such that careful interpretation of their significance is necessary.

Original languageEnglish
JournalAlcoholism: Clinical and Experimental Research
Volume25
Issue number6 SUPPL.
Publication statusPublished - 2001

Fingerprint

Alcohol Dehydrogenase
Helicobacter Infections
Gastric Mucosa
Human Activities
Helicobacter pylori
Stomach
Acetaldehyde
Gastritis
NAD
Formaldehyde
Isoenzymes
Oxidation
Substrates
Human engineering
Mucous Membrane
alcohol dehydrogenase IV
Population
formaldehyde dehydrogenase (glutathione)
Proteins

Keywords

  • Alcohol Dehydrogenase
  • Stomach

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Alcohol dehydrogenase activities in the human gastric mucosa : Effects of Helicobacter pylori infection, sex, age, and the part of the stomach. / Matsumoto, Michinaga; Yokoyama, Hirokazu; Shiraishi, Haruko; Suzuki, Hidekazu; Kato, Shinzo; Miura, Souichiro; Ishii, Hiromasa.

In: Alcoholism: Clinical and Experimental Research, Vol. 25, No. 6 SUPPL., 2001.

Research output: Contribution to journalArticle

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abstract = "Background: Human gastric mucosa contains three alcohol dehydrogenase (ADH) isozymes (classes I, III, and IV). Various factors such as Helicobacter pylori infection, sex, age, and the part of the stomach involved have been suggested to affect alcohol dehydrogenase activities, although these views are controversial. In this study, these unsettled issues were reexamined. Methods: Activities of class I and IV ADHs were evaluated in the cytosolic fraction of human gastric mucosa samples by reduction of their preferred substrates, namely acetaldehyde and m-nitrobenzaldehyde, and activities of class III were evaluated by oxidation of its preferred substrate, formaldehyde. Then, effects of Helicobacter pylori infection, sex, age, and the part of the stomach involved were examined. Results: Class I, III, and IV ADH activities were 17.5 ± 8.4, 4.2 ± 2.5, and 8.9 ± 3.9 nmol of nicotinamide adenine dinucleotide oxidation per minute per milligram of protein, respectively, for the entire population. Helicobacter pylori infection significantly reduced class I and IV ADH activities but did not affect activity of class III. In the samples without Helicobacter pylori infection and severe gastritis, sex did not affect class I, III, or IV ADH activities. In the same series, class IV ADH activity significantly decreased with age (p = 0.006), whereas no correlation was found between age and ADH activity of class I and III ADHs. The level of class IV ADH activity was significantly higher in the upper body than in the lower regions, whereas no such heterogeneity was observed in class I and III ADH. Conclusions: Various factors affect human gastric ADH activities, such that careful interpretation of their significance is necessary.",
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AU - Suzuki, Hidekazu

AU - Kato, Shinzo

AU - Miura, Souichiro

AU - Ishii, Hiromasa

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N2 - Background: Human gastric mucosa contains three alcohol dehydrogenase (ADH) isozymes (classes I, III, and IV). Various factors such as Helicobacter pylori infection, sex, age, and the part of the stomach involved have been suggested to affect alcohol dehydrogenase activities, although these views are controversial. In this study, these unsettled issues were reexamined. Methods: Activities of class I and IV ADHs were evaluated in the cytosolic fraction of human gastric mucosa samples by reduction of their preferred substrates, namely acetaldehyde and m-nitrobenzaldehyde, and activities of class III were evaluated by oxidation of its preferred substrate, formaldehyde. Then, effects of Helicobacter pylori infection, sex, age, and the part of the stomach involved were examined. Results: Class I, III, and IV ADH activities were 17.5 ± 8.4, 4.2 ± 2.5, and 8.9 ± 3.9 nmol of nicotinamide adenine dinucleotide oxidation per minute per milligram of protein, respectively, for the entire population. Helicobacter pylori infection significantly reduced class I and IV ADH activities but did not affect activity of class III. In the samples without Helicobacter pylori infection and severe gastritis, sex did not affect class I, III, or IV ADH activities. In the same series, class IV ADH activity significantly decreased with age (p = 0.006), whereas no correlation was found between age and ADH activity of class I and III ADHs. The level of class IV ADH activity was significantly higher in the upper body than in the lower regions, whereas no such heterogeneity was observed in class I and III ADH. Conclusions: Various factors affect human gastric ADH activities, such that careful interpretation of their significance is necessary.

AB - Background: Human gastric mucosa contains three alcohol dehydrogenase (ADH) isozymes (classes I, III, and IV). Various factors such as Helicobacter pylori infection, sex, age, and the part of the stomach involved have been suggested to affect alcohol dehydrogenase activities, although these views are controversial. In this study, these unsettled issues were reexamined. Methods: Activities of class I and IV ADHs were evaluated in the cytosolic fraction of human gastric mucosa samples by reduction of their preferred substrates, namely acetaldehyde and m-nitrobenzaldehyde, and activities of class III were evaluated by oxidation of its preferred substrate, formaldehyde. Then, effects of Helicobacter pylori infection, sex, age, and the part of the stomach involved were examined. Results: Class I, III, and IV ADH activities were 17.5 ± 8.4, 4.2 ± 2.5, and 8.9 ± 3.9 nmol of nicotinamide adenine dinucleotide oxidation per minute per milligram of protein, respectively, for the entire population. Helicobacter pylori infection significantly reduced class I and IV ADH activities but did not affect activity of class III. In the samples without Helicobacter pylori infection and severe gastritis, sex did not affect class I, III, or IV ADH activities. In the same series, class IV ADH activity significantly decreased with age (p = 0.006), whereas no correlation was found between age and ADH activity of class I and III ADHs. The level of class IV ADH activity was significantly higher in the upper body than in the lower regions, whereas no such heterogeneity was observed in class I and III ADH. Conclusions: Various factors affect human gastric ADH activities, such that careful interpretation of their significance is necessary.

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