Alcohol, one-carbon nutrient intake, and risk of colorectal cancer according to tumor methylation level of IGF2 differentially methylated region

Reiko Nishihara, Molin Wang, Zhi Rong Qian, Yoshifumi Baba, Mai Yamauchi, Kosuke Mima, Yasutaka Sukawa, Sun A. Kim, Kentaro Inamura, Xuehong Zhang, Kana Wu, Edward L. Giovannucci, Andrew T. Chan, Charles S. Fuchs, Shuji Ogino, Eva S. Schernhammer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Although a higher consumption of alcohol, which is a methyl-group antagonist, was previously associated with colorectal cancer risk, mechanisms remain poorly understood.

Objective: We hypothesized that excess alcohol consumption might increase risk of colorectal carcinoma with hypomethylation of insulinlike growth factor 2 (IGF2) differentially methylated region-0 (DMR0), which was previously associated with a worse prognosis.

Design: With the use of a molecular pathologic epidemiology database in 2 prospective cohort studies, the Nurses' Health Study and Health Professionals Follow-up Study, we examined the association between alcohol intake and incident colorectal cancer according to the tumor methylation level of IGF2 DMR0. Duplication-method Cox proportional cause-specific hazards regression for competing risk data were used to compute HRs and 95% CIs. In addition, we investigated intakes of vitamin B-6, vitamin B-12, methionine, and folate as exposures.

Results: During 3,206,985 person-years of follow-up, we identified 993 rectal and colon cancer cases with an available tumor DNA methylation status. Compared with no alcohol consumption, the consumption of >15 g alcohol/d was associated with elevated risk of colorectal cancer with lower levels of IGF2 DMR0 methylation [within the first and second quartiles: HRs of 1.55 (95% CI: 1.08, 2.24) and 2.11 (95% CI: 1.44, 3.07), respectively]. By contrast, alcohol consumption was not associated with cancer with higher levels of IGF2 DMR0 methylation. The association between alcohol and cancer risk differed significantly by IGF2 DMR0 methylation level (P-heterogeneity = 0.006). The association of vitamin B-6, vitamin B-12, and folate intakes with cancer risk did not significantly differ according to IGF2 DMR0 methylation level (P-heterogeneity . 0.2).

Conclusions: Higher alcohol consumption was associated with risk of colorectal cancer with IGF2 DMR0 hypomethylation but not risk of cancer with high-level IGF2 DMR0 methylation. The association between alcohol intake and colorectal cancer risk may differ by tumor epigenetic features.

Original languageEnglish
Pages (from-to)1479-1488
Number of pages10
JournalAmerican Journal of Clinical Nutrition
Volume100
Issue number6
DOIs
Publication statusPublished - 2014 Dec 1
Externally publishedYes

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Methylation
Colorectal Neoplasms
Intercellular Signaling Peptides and Proteins
Carbon
Alcohols
Food
Alcohol Drinking
Neoplasms
Vitamin B Complex
Vitamin B 6
Vitamin B 12
Folic Acid
Molecular Epidemiology
Health
DNA Methylation
Rectal Neoplasms
Epigenomics
Methionine
Colonic Neoplasms
Cohort Studies

Keywords

  • Biomarker
  • Epigenetics
  • Imprinting
  • Molecular pathological epidemiology
  • One carbon metabolism

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Alcohol, one-carbon nutrient intake, and risk of colorectal cancer according to tumor methylation level of IGF2 differentially methylated region. / Nishihara, Reiko; Wang, Molin; Qian, Zhi Rong; Baba, Yoshifumi; Yamauchi, Mai; Mima, Kosuke; Sukawa, Yasutaka; Kim, Sun A.; Inamura, Kentaro; Zhang, Xuehong; Wu, Kana; Giovannucci, Edward L.; Chan, Andrew T.; Fuchs, Charles S.; Ogino, Shuji; Schernhammer, Eva S.

In: American Journal of Clinical Nutrition, Vol. 100, No. 6, 01.12.2014, p. 1479-1488.

Research output: Contribution to journalArticle

Nishihara, R, Wang, M, Qian, ZR, Baba, Y, Yamauchi, M, Mima, K, Sukawa, Y, Kim, SA, Inamura, K, Zhang, X, Wu, K, Giovannucci, EL, Chan, AT, Fuchs, CS, Ogino, S & Schernhammer, ES 2014, 'Alcohol, one-carbon nutrient intake, and risk of colorectal cancer according to tumor methylation level of IGF2 differentially methylated region', American Journal of Clinical Nutrition, vol. 100, no. 6, pp. 1479-1488. https://doi.org/10.3945/ajcn.114.095539
Nishihara, Reiko ; Wang, Molin ; Qian, Zhi Rong ; Baba, Yoshifumi ; Yamauchi, Mai ; Mima, Kosuke ; Sukawa, Yasutaka ; Kim, Sun A. ; Inamura, Kentaro ; Zhang, Xuehong ; Wu, Kana ; Giovannucci, Edward L. ; Chan, Andrew T. ; Fuchs, Charles S. ; Ogino, Shuji ; Schernhammer, Eva S. / Alcohol, one-carbon nutrient intake, and risk of colorectal cancer according to tumor methylation level of IGF2 differentially methylated region. In: American Journal of Clinical Nutrition. 2014 ; Vol. 100, No. 6. pp. 1479-1488.
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abstract = "Background: Although a higher consumption of alcohol, which is a methyl-group antagonist, was previously associated with colorectal cancer risk, mechanisms remain poorly understood.Objective: We hypothesized that excess alcohol consumption might increase risk of colorectal carcinoma with hypomethylation of insulinlike growth factor 2 (IGF2) differentially methylated region-0 (DMR0), which was previously associated with a worse prognosis.Design: With the use of a molecular pathologic epidemiology database in 2 prospective cohort studies, the Nurses' Health Study and Health Professionals Follow-up Study, we examined the association between alcohol intake and incident colorectal cancer according to the tumor methylation level of IGF2 DMR0. Duplication-method Cox proportional cause-specific hazards regression for competing risk data were used to compute HRs and 95{\%} CIs. In addition, we investigated intakes of vitamin B-6, vitamin B-12, methionine, and folate as exposures.Results: During 3,206,985 person-years of follow-up, we identified 993 rectal and colon cancer cases with an available tumor DNA methylation status. Compared with no alcohol consumption, the consumption of >15 g alcohol/d was associated with elevated risk of colorectal cancer with lower levels of IGF2 DMR0 methylation [within the first and second quartiles: HRs of 1.55 (95{\%} CI: 1.08, 2.24) and 2.11 (95{\%} CI: 1.44, 3.07), respectively]. By contrast, alcohol consumption was not associated with cancer with higher levels of IGF2 DMR0 methylation. The association between alcohol and cancer risk differed significantly by IGF2 DMR0 methylation level (P-heterogeneity = 0.006). The association of vitamin B-6, vitamin B-12, and folate intakes with cancer risk did not significantly differ according to IGF2 DMR0 methylation level (P-heterogeneity . 0.2).Conclusions: Higher alcohol consumption was associated with risk of colorectal cancer with IGF2 DMR0 hypomethylation but not risk of cancer with high-level IGF2 DMR0 methylation. The association between alcohol intake and colorectal cancer risk may differ by tumor epigenetic features.",
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author = "Reiko Nishihara and Molin Wang and Qian, {Zhi Rong} and Yoshifumi Baba and Mai Yamauchi and Kosuke Mima and Yasutaka Sukawa and Kim, {Sun A.} and Kentaro Inamura and Xuehong Zhang and Kana Wu and Giovannucci, {Edward L.} and Chan, {Andrew T.} and Fuchs, {Charles S.} and Shuji Ogino and Schernhammer, {Eva S.}",
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T1 - Alcohol, one-carbon nutrient intake, and risk of colorectal cancer according to tumor methylation level of IGF2 differentially methylated region

AU - Nishihara, Reiko

AU - Wang, Molin

AU - Qian, Zhi Rong

AU - Baba, Yoshifumi

AU - Yamauchi, Mai

AU - Mima, Kosuke

AU - Sukawa, Yasutaka

AU - Kim, Sun A.

AU - Inamura, Kentaro

AU - Zhang, Xuehong

AU - Wu, Kana

AU - Giovannucci, Edward L.

AU - Chan, Andrew T.

AU - Fuchs, Charles S.

AU - Ogino, Shuji

AU - Schernhammer, Eva S.

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Background: Although a higher consumption of alcohol, which is a methyl-group antagonist, was previously associated with colorectal cancer risk, mechanisms remain poorly understood.Objective: We hypothesized that excess alcohol consumption might increase risk of colorectal carcinoma with hypomethylation of insulinlike growth factor 2 (IGF2) differentially methylated region-0 (DMR0), which was previously associated with a worse prognosis.Design: With the use of a molecular pathologic epidemiology database in 2 prospective cohort studies, the Nurses' Health Study and Health Professionals Follow-up Study, we examined the association between alcohol intake and incident colorectal cancer according to the tumor methylation level of IGF2 DMR0. Duplication-method Cox proportional cause-specific hazards regression for competing risk data were used to compute HRs and 95% CIs. In addition, we investigated intakes of vitamin B-6, vitamin B-12, methionine, and folate as exposures.Results: During 3,206,985 person-years of follow-up, we identified 993 rectal and colon cancer cases with an available tumor DNA methylation status. Compared with no alcohol consumption, the consumption of >15 g alcohol/d was associated with elevated risk of colorectal cancer with lower levels of IGF2 DMR0 methylation [within the first and second quartiles: HRs of 1.55 (95% CI: 1.08, 2.24) and 2.11 (95% CI: 1.44, 3.07), respectively]. By contrast, alcohol consumption was not associated with cancer with higher levels of IGF2 DMR0 methylation. The association between alcohol and cancer risk differed significantly by IGF2 DMR0 methylation level (P-heterogeneity = 0.006). The association of vitamin B-6, vitamin B-12, and folate intakes with cancer risk did not significantly differ according to IGF2 DMR0 methylation level (P-heterogeneity . 0.2).Conclusions: Higher alcohol consumption was associated with risk of colorectal cancer with IGF2 DMR0 hypomethylation but not risk of cancer with high-level IGF2 DMR0 methylation. The association between alcohol intake and colorectal cancer risk may differ by tumor epigenetic features.

AB - Background: Although a higher consumption of alcohol, which is a methyl-group antagonist, was previously associated with colorectal cancer risk, mechanisms remain poorly understood.Objective: We hypothesized that excess alcohol consumption might increase risk of colorectal carcinoma with hypomethylation of insulinlike growth factor 2 (IGF2) differentially methylated region-0 (DMR0), which was previously associated with a worse prognosis.Design: With the use of a molecular pathologic epidemiology database in 2 prospective cohort studies, the Nurses' Health Study and Health Professionals Follow-up Study, we examined the association between alcohol intake and incident colorectal cancer according to the tumor methylation level of IGF2 DMR0. Duplication-method Cox proportional cause-specific hazards regression for competing risk data were used to compute HRs and 95% CIs. In addition, we investigated intakes of vitamin B-6, vitamin B-12, methionine, and folate as exposures.Results: During 3,206,985 person-years of follow-up, we identified 993 rectal and colon cancer cases with an available tumor DNA methylation status. Compared with no alcohol consumption, the consumption of >15 g alcohol/d was associated with elevated risk of colorectal cancer with lower levels of IGF2 DMR0 methylation [within the first and second quartiles: HRs of 1.55 (95% CI: 1.08, 2.24) and 2.11 (95% CI: 1.44, 3.07), respectively]. By contrast, alcohol consumption was not associated with cancer with higher levels of IGF2 DMR0 methylation. The association between alcohol and cancer risk differed significantly by IGF2 DMR0 methylation level (P-heterogeneity = 0.006). The association of vitamin B-6, vitamin B-12, and folate intakes with cancer risk did not significantly differ according to IGF2 DMR0 methylation level (P-heterogeneity . 0.2).Conclusions: Higher alcohol consumption was associated with risk of colorectal cancer with IGF2 DMR0 hypomethylation but not risk of cancer with high-level IGF2 DMR0 methylation. The association between alcohol intake and colorectal cancer risk may differ by tumor epigenetic features.

KW - Biomarker

KW - Epigenetics

KW - Imprinting

KW - Molecular pathological epidemiology

KW - One carbon metabolism

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