Aldolase-Catalyzed C_C Bond Formation of Carbohydrate Synthesis

Takeshi Sugai, K. Fuhshuku

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Three types of aldolase-catalyzed reactions which are promising for C. C bond formation, not only for the practical synthesis of carbohydrates but also in active pharmaceutical ingredients manufacturing, were introduced. The authors focused on the use of three nonphosphorylated nucleophiles: pyruvic acid, acetaldehyde, and dihydroxyacetone. Furthermore, nitroaldol reaction catalyzed by hydroxynitrile lyase (HNL) is noted as an example of catalysis caused by the promiscuous ability of enzymes. All of following the aspects involving screening of new enzymes, characterization, substrate specificity study, synthetic application, and protein engineering are very important in developing improved biocatalytic processes.

Original languageEnglish
Title of host publicationComprehensive Organic Synthesis: Second Edition
PublisherElsevier Ltd.
Pages512-522
Number of pages11
Volume2
ISBN (Print)9780080977430
DOIs
Publication statusPublished - 2014 Feb

Fingerprint

Fructose-Bisphosphate Aldolase
mandelonitrile lyase
Carbohydrates
Enzymes
Dihydroxyacetone
Nucleophiles
Acetaldehyde
Pyruvic Acid
Drug products
Catalysis
Screening
Proteins
Acids
Substrates
Pharmaceutical Preparations
nitroaldol

Keywords

  • 3-deoxy-d-manno-2-octusolonic acid aldolase
  • Aldolase BphI
  • Deoxyribose-5-phosphate aldolase
  • Fructose-6-phosphate aldolase
  • Hydroxynitrile lyase
  • NAL
  • Sialic acid aldolase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Sugai, T., & Fuhshuku, K. (2014). Aldolase-Catalyzed C_C Bond Formation of Carbohydrate Synthesis. In Comprehensive Organic Synthesis: Second Edition (Vol. 2, pp. 512-522). Elsevier Ltd.. https://doi.org/10.1016/B978-0-08-097742-3.00212-3

Aldolase-Catalyzed C_C Bond Formation of Carbohydrate Synthesis. / Sugai, Takeshi; Fuhshuku, K.

Comprehensive Organic Synthesis: Second Edition. Vol. 2 Elsevier Ltd., 2014. p. 512-522.

Research output: Chapter in Book/Report/Conference proceedingChapter

Sugai, T & Fuhshuku, K 2014, Aldolase-Catalyzed C_C Bond Formation of Carbohydrate Synthesis. in Comprehensive Organic Synthesis: Second Edition. vol. 2, Elsevier Ltd., pp. 512-522. https://doi.org/10.1016/B978-0-08-097742-3.00212-3
Sugai T, Fuhshuku K. Aldolase-Catalyzed C_C Bond Formation of Carbohydrate Synthesis. In Comprehensive Organic Synthesis: Second Edition. Vol. 2. Elsevier Ltd. 2014. p. 512-522 https://doi.org/10.1016/B978-0-08-097742-3.00212-3
Sugai, Takeshi ; Fuhshuku, K. / Aldolase-Catalyzed C_C Bond Formation of Carbohydrate Synthesis. Comprehensive Organic Synthesis: Second Edition. Vol. 2 Elsevier Ltd., 2014. pp. 512-522
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