Aldolase-Catalyzed C_C Bond Formation of Carbohydrate Synthesis

T. Sugai, K. Fuhshuku

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Three types of aldolase-catalyzed reactions which are promising for C. C bond formation, not only for the practical synthesis of carbohydrates but also in active pharmaceutical ingredients manufacturing, were introduced. The authors focused on the use of three nonphosphorylated nucleophiles: pyruvic acid, acetaldehyde, and dihydroxyacetone. Furthermore, nitroaldol reaction catalyzed by hydroxynitrile lyase (HNL) is noted as an example of catalysis caused by the promiscuous ability of enzymes. All of following the aspects involving screening of new enzymes, characterization, substrate specificity study, synthetic application, and protein engineering are very important in developing improved biocatalytic processes.

Original languageEnglish
Title of host publicationComprehensive Organic Synthesis
Subtitle of host publicationSecond Edition
PublisherElsevier Ltd
Pages512-522
Number of pages11
Volume2
ISBN (Print)9780080977430
DOIs
Publication statusPublished - 2014 Feb 1

Keywords

  • 3-deoxy-d-manno-2-octusolonic acid aldolase
  • Aldolase BphI
  • Deoxyribose-5-phosphate aldolase
  • Fructose-6-phosphate aldolase
  • Hydroxynitrile lyase
  • NAL
  • Sialic acid aldolase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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  • Cite this

    Sugai, T., & Fuhshuku, K. (2014). Aldolase-Catalyzed C_C Bond Formation of Carbohydrate Synthesis. In Comprehensive Organic Synthesis: Second Edition (Vol. 2, pp. 512-522). Elsevier Ltd. https://doi.org/10.1016/B978-0-08-097742-3.00212-3