Aldose reductase inhibition and the phosphorus-31 profile of the intact diabetic rat lens

Kazuo Tsubota, M. Yoshida, T. Toda, M. Ono, K. Kajiwara, H. M. Cheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Diabetic metabolic change and response to aldose reductase inhibition in the Wistar rat lens were examined with phosphorus-31 (31p) nuclear magnetic resonance (NMR) spectroscopy. To avoid artifacts in sample preparation, we used freshly excised lenses and acquired NMR data for 20 min immediately after lens extraction. The results showed a diabetes-induced time-dependent loss of ATP and phosphorylcholine (PC), an increase in α-glycerophosphate (α-GP) and inorganic phosphate and the appearance of sorbitol-3-phosphate (S-3-P) and fructose-3-phosphate (F-3-P). Oral but not topical dosing of an aldose reductase inhibitor, 5-(3-ethoxy-4-pentyloxyphenyl)2,4-thiazolidinedione, resulted in a positive dose-response correlation characterized by a restoration of PC, S-3-P and F-3-P to the prediabetic level; however, α-GP and ATP were only partially normalized. The significance of the 31p change was further discussed.

Original languageEnglish
Pages (from-to)393-399
Number of pages7
JournalOphthalmic Research
Volume25
Issue number6
Publication statusPublished - 1993

Fingerprint

Aldehyde Reductase
Phosphorus
Lenses
Phosphorylcholine
Magnetic Resonance Spectroscopy
Adenosine Triphosphate
Glycerophosphates
Artifacts
Wistar Rats
Phosphates
sorbitol 3-phosphate
fructose 3-phosphate

Keywords

  • P nuclear magnetic resonance spectroscopy
  • Adenosine 5'-triphosphate
  • Aldose reductase inhibitor
  • Diabetic rat lens
  • Fructose-3-phosphate
  • Sorbitol pathway
  • Sorbitol-3-phosphate
  • Wistar rat

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Tsubota, K., Yoshida, M., Toda, T., Ono, M., Kajiwara, K., & Cheng, H. M. (1993). Aldose reductase inhibition and the phosphorus-31 profile of the intact diabetic rat lens. Ophthalmic Research, 25(6), 393-399.

Aldose reductase inhibition and the phosphorus-31 profile of the intact diabetic rat lens. / Tsubota, Kazuo; Yoshida, M.; Toda, T.; Ono, M.; Kajiwara, K.; Cheng, H. M.

In: Ophthalmic Research, Vol. 25, No. 6, 1993, p. 393-399.

Research output: Contribution to journalArticle

Tsubota, K, Yoshida, M, Toda, T, Ono, M, Kajiwara, K & Cheng, HM 1993, 'Aldose reductase inhibition and the phosphorus-31 profile of the intact diabetic rat lens', Ophthalmic Research, vol. 25, no. 6, pp. 393-399.
Tsubota, Kazuo ; Yoshida, M. ; Toda, T. ; Ono, M. ; Kajiwara, K. ; Cheng, H. M. / Aldose reductase inhibition and the phosphorus-31 profile of the intact diabetic rat lens. In: Ophthalmic Research. 1993 ; Vol. 25, No. 6. pp. 393-399.
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