Aldose reductase inhibition and thephosphorus-31 profile of the intact diabetic rat lens

Kazuo Tsubota, Masaaki Yoshida, Takashi Toda, Masafumi Ono, Kazuto Kajiwara, Hong Ming Cheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Diabetic metabolic change and response to aldose reductase inhibition in the Wistar rat lens were examined with phospho-rus-31 (31P) nuclear magnetic resonance (NMR) spectroscopy. To avoid artifacts in sample preparation, we used freshly excised lenses and acquired NMR data for 20 min immediately after lens extraction. The results showed a diabetes-induced time-dependent loss of ATP and phosphorylcholine (PC), an increase in a-glycerophosphate (ct-GP) and inorganic phosphate and the appearance of sorbitol-3-phosphate (S-3-P) and fructose-3-phosphate (F-3-P). Oral but not topical dosing of an aldose reductase inhibitor, 5-(3-ethoxy-4-pentyloxyphenyl)-2,4-thiazolidinedione, resulted in a positive dose-response correlation characterized by a restoration of PC, S-3-P and F-3-P to the prediabetic level; however, a-GP and ATP were only partially normalized. The significance of the 31P change was further discussed.

Original languageEnglish
Pages (from-to)393-399
Number of pages7
JournalOphthalmic research
Volume25
Issue number6
DOIs
Publication statusPublished - 1993 Jan 1

Keywords

  • 31P nuclear magnetic resonance spectroscopy
  • Adenosine 5'-triphosphate
  • Diabetic rat lens
  • Fructose-3-phosphate
  • Sorbitol pathway
  • Sorbitol-3-phosphate
  • Wistar rat aldose reductase inhibitor

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Aldose reductase inhibition and thephosphorus-31 profile of the intact diabetic rat lens'. Together they form a unique fingerprint.

  • Cite this