Aldosterone-induced kidney injury is mediated by NFκB activation

Seiichi Fukuda, Chihiro Horimai, Kaori Harada, Toshifumi Wakamatsu, Hiroshi Fukasawa, Susumu Muto, Akiko Itai, Matsuhiko Hayashi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Aldosterone induces inflammation and fibrosis in the kidney, while nuclear factor κB (NFκB) plays key roles in inflammation mediated by various cytokines. Here, we determined the roles of NFκB activation in aldosterone-induced kidney injury. Methods: We used unilaterally nephrectomized rats with or without continuous aldosterone infusion and 0.9% saline as drinking water for 3 weeks. IMD-1041, an IKKβ inhibitor, and spironolactone were orally administered to inhibit NFκB and mineralocorticoid receptor, respectively. Results: The aldosterone-infused rats exhibited severe kidney injury, hypertension, and increased expression of pro-inflammatory and fibrotic proteins, osteopontin, fibrinogen, collagen type I, and PAI-1. Western blotting confirmed NFκB activation by aldosterone by the increased amount of p65 in the nuclear fraction of the kidney, and oral IMD-1041 prevented the kidney injury and lessened the increase in pro-inflammatory and fibrotic proteins without significant changes in blood pressures. In addition, changes in angiotensin-converting enzyme 2 (ACE2), which has been found to act as a protective factor in various kidney injury models, were examined. Immunofluorescence studies revealed the presence of ACE2 in the brush-border membrane of the proximal convoluted tubules and markedly blunted ACE2 staining in aldosterone-infused rats. The decrease in amount of ACE2 protein was confirmed by Western blotting, and IMD-1041 also prevented the decrease in ACE2. The administration of spironolactone also abolished the effects of aldosterone. Conclusion: Our results suggest that aldosterone induces kidney injury via activation of NFκB and mineralocorticoid receptor, and that decreased ACE2 expression may play an important role in aldosterone-induced kidney injury.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalClinical and Experimental Nephrology
Volume15
Issue number1
DOIs
Publication statusPublished - 2011 Feb

Fingerprint

Aldosterone
Kidney
Wounds and Injuries
Mineralocorticoid Receptors
Spironolactone
Western Blotting
Inflammation
Proteins
Osteopontin
Plasminogen Activator Inhibitor 1
Microvilli
Collagen Type I
Drinking Water
Fibrinogen
Fluorescent Antibody Technique
angiotensin converting enzyme 2
Fibrosis
Staining and Labeling
Cytokines
Blood Pressure

Keywords

  • Aldosterone
  • Cytokines
  • Inflammation
  • Progression of chronic renal failure

ASJC Scopus subject areas

  • Nephrology
  • Physiology
  • Physiology (medical)

Cite this

Fukuda, S., Horimai, C., Harada, K., Wakamatsu, T., Fukasawa, H., Muto, S., ... Hayashi, M. (2011). Aldosterone-induced kidney injury is mediated by NFκB activation. Clinical and Experimental Nephrology, 15(1), 41-49. https://doi.org/10.1007/s10157-010-0373-1

Aldosterone-induced kidney injury is mediated by NFκB activation. / Fukuda, Seiichi; Horimai, Chihiro; Harada, Kaori; Wakamatsu, Toshifumi; Fukasawa, Hiroshi; Muto, Susumu; Itai, Akiko; Hayashi, Matsuhiko.

In: Clinical and Experimental Nephrology, Vol. 15, No. 1, 02.2011, p. 41-49.

Research output: Contribution to journalArticle

Fukuda, S, Horimai, C, Harada, K, Wakamatsu, T, Fukasawa, H, Muto, S, Itai, A & Hayashi, M 2011, 'Aldosterone-induced kidney injury is mediated by NFκB activation', Clinical and Experimental Nephrology, vol. 15, no. 1, pp. 41-49. https://doi.org/10.1007/s10157-010-0373-1
Fukuda S, Horimai C, Harada K, Wakamatsu T, Fukasawa H, Muto S et al. Aldosterone-induced kidney injury is mediated by NFκB activation. Clinical and Experimental Nephrology. 2011 Feb;15(1):41-49. https://doi.org/10.1007/s10157-010-0373-1
Fukuda, Seiichi ; Horimai, Chihiro ; Harada, Kaori ; Wakamatsu, Toshifumi ; Fukasawa, Hiroshi ; Muto, Susumu ; Itai, Akiko ; Hayashi, Matsuhiko. / Aldosterone-induced kidney injury is mediated by NFκB activation. In: Clinical and Experimental Nephrology. 2011 ; Vol. 15, No. 1. pp. 41-49.
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AB - Background: Aldosterone induces inflammation and fibrosis in the kidney, while nuclear factor κB (NFκB) plays key roles in inflammation mediated by various cytokines. Here, we determined the roles of NFκB activation in aldosterone-induced kidney injury. Methods: We used unilaterally nephrectomized rats with or without continuous aldosterone infusion and 0.9% saline as drinking water for 3 weeks. IMD-1041, an IKKβ inhibitor, and spironolactone were orally administered to inhibit NFκB and mineralocorticoid receptor, respectively. Results: The aldosterone-infused rats exhibited severe kidney injury, hypertension, and increased expression of pro-inflammatory and fibrotic proteins, osteopontin, fibrinogen, collagen type I, and PAI-1. Western blotting confirmed NFκB activation by aldosterone by the increased amount of p65 in the nuclear fraction of the kidney, and oral IMD-1041 prevented the kidney injury and lessened the increase in pro-inflammatory and fibrotic proteins without significant changes in blood pressures. In addition, changes in angiotensin-converting enzyme 2 (ACE2), which has been found to act as a protective factor in various kidney injury models, were examined. Immunofluorescence studies revealed the presence of ACE2 in the brush-border membrane of the proximal convoluted tubules and markedly blunted ACE2 staining in aldosterone-infused rats. The decrease in amount of ACE2 protein was confirmed by Western blotting, and IMD-1041 also prevented the decrease in ACE2. The administration of spironolactone also abolished the effects of aldosterone. Conclusion: Our results suggest that aldosterone induces kidney injury via activation of NFκB and mineralocorticoid receptor, and that decreased ACE2 expression may play an important role in aldosterone-induced kidney injury.

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