Aligned free-standing muscle fibers connected with neurons

Y. Morimoto, M. Kato-Negishi, Hiroaki Onoe, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We propose aligned free-standing skeletal muscle fibers connecting with neurons. By confining skeletal cells, C2C12, with collagen gel into polydimethylsiloxane (PDMS) trenches, we successfully formed the muscle fibers that are highly oriented to the fiber direction. We cultured the free-standing muscle fibers maintaining their tension since the edge of the fibers was fixed to glass substrates. Furthermore, we can culture the muscle fibers with rat primary neurons, and the neurons successfully connect with the muscle fibers. We believe that the muscle fibers connected with the neurons have a potential to contract by the nerve impulse.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages789-791
Number of pages3
Volume2
Publication statusPublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Neurons
Muscle
Fibers
Polydimethylsiloxane
Collagen
Rats
Gels
Glass
Substrates

Keywords

  • 3D tissue engineering
  • Muscle contraction
  • Muscle fiber
  • Neuron-muscle co-culture
  • PDMS stamp

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Morimoto, Y., Kato-Negishi, M., Onoe, H., & Takeuchi, S. (2011). Aligned free-standing muscle fibers connected with neurons. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 2, pp. 789-791)

Aligned free-standing muscle fibers connected with neurons. / Morimoto, Y.; Kato-Negishi, M.; Onoe, Hiroaki; Takeuchi, S.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. p. 789-791.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Morimoto, Y, Kato-Negishi, M, Onoe, H & Takeuchi, S 2011, Aligned free-standing muscle fibers connected with neurons. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 2, pp. 789-791, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.
Morimoto Y, Kato-Negishi M, Onoe H, Takeuchi S. Aligned free-standing muscle fibers connected with neurons. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2. 2011. p. 789-791
Morimoto, Y. ; Kato-Negishi, M. ; Onoe, Hiroaki ; Takeuchi, S. / Aligned free-standing muscle fibers connected with neurons. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. pp. 789-791
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