Aligned free-standing muscle fibers connected with neurons

Y. Morimoto, M. Kato-Negishi, H. Onoe, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We propose aligned free-standing skeletal muscle fibers connecting with neurons. By confining skeletal cells, C2C12, with collagen gel into polydimethylsiloxane (PDMS) trenches, we successfully formed the muscle fibers that are highly oriented to the fiber direction. We cultured the free-standing muscle fibers maintaining their tension since the edge of the fibers was fixed to glass substrates. Furthermore, we can culture the muscle fibers with rat primary neurons, and the neurons successfully connect with the muscle fibers. We believe that the muscle fibers connected with the neurons have a potential to contract by the nerve impulse.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages789-791
Number of pages3
Publication statusPublished - 2011 Dec 1
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • 3D tissue engineering
  • Muscle contraction
  • Muscle fiber
  • Neuron-muscle co-culture
  • PDMS stamp

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Morimoto, Y., Kato-Negishi, M., Onoe, H., & Takeuchi, S. (2011). Aligned free-standing muscle fibers connected with neurons. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 789-791). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).