Alignment-based approach for durable data storage into living organisms

Nozomu Yachie, Kazuhide Sekiyama, Junichi Sugahara, Yoshiaki Ohashi, Masaru Tomita

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The practical realization of DNA data storage is a major scientific goal. Here we introduce a simple, flexible, and robust data storage and retrieval method based on sequence alignment of the genomic DNA of living organisms. Duplicated data encoded by different oligonucleotide sequences was inserted redundantly into multiple loci of the Bacillus subtilis genome. Multiple alignment of the bit data sequences decoded by B. subtilis genome sequences enabled the retrieval of stable and compact data without the need for template DNA, parity checks, or error-correcting algorithms. Combined with the computational simulation of data retrieval from mutated message DNA, a practical use of this alignment-based method is discussed.

Original languageEnglish
Pages (from-to)501-505
Number of pages5
JournalBiotechnology Progress
Volume23
Issue number2
DOIs
Publication statusPublished - 2007 Mar 1

ASJC Scopus subject areas

  • Biotechnology

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