Alimentary tract and pancreas. Bile acid-induced depolarization of mitochondrial membrane potential preceding cell injury in cultured gastric mucosal cells

S. Miura, D. Fukumura, H. Shiozaki, M. Suzuki, I. Kurose, M. Suematsu, M. Tsuchiya, H. Ishii

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Changes in energy metabolism elicited by sodium taurocholate and their relation to cell viability were determined in gastric mucosal cells. Cultured mucosal cells were labelled with rhodamine-123, a mitochondrial energization-sensitive fluorescence probe, or by propidium iodide, a fluorochrome which labels the nuclei of non-viable cells. The cells were observed under a fluorescence microscope with a laser scanning confocal imaging system. After the addition of sodium taurocholate at concentrations >5 mol/L, mucosal cells showed a rapid and significant decrease in rhodamine-123 fluorescence. A decrease to 40% of the pretreated values at 30 min was seen with a concentration of sodium taurocholate of 7.5 mmol/L. A marked increase in the percentage of propidium iodide-positive cells was noted when the concentration of sodium taurocholate exceeded 5 mmol/L. However, the extent of the decrease in rhodamine-123 fluorescence was always greater than the increase in the percentage of propidium iodide-positive cells, suggesting that most of these gastric mucosal cells remained viable. It is therefore suggested that the decrease in rhodamine-123 fluorescence is largely due to the disturbed oxidative phosphorylation of mitochondria. Pretreatment of gastric mucosal cells with low concentrations of ethanol resulted in a significant cytoprotective effect against sodium taurocholate injury with significant prevention of a decrease in rhodamine-123 fluoresence. It is concluded that sodium taurocholate induces a depolarization of the mitochondrial membrane potential preceding cell injury and that the cytoprotective effect of ethanol relates to its attenuation of the uncoupling effect.

Original languageEnglish
Pages (from-to)621-626
Number of pages6
JournalJournal of Gastroenterology and Hepatology (Australia)
Issue number6
Publication statusPublished - 1995



  • Adaptive cytoprotection
  • Bile acid
  • Cell death
  • Gastric cell culture
  • Laser scanning confocal imaging system
  • Mitochondria
  • Propidium iodide
  • Rhodamine 123

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

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