Alimentary tract and pancreas. Bile acid-induced depolarization of mitochondrial membrane potential preceding cell injury in cultured gastric mucosal cells

S. Miura, D. Fukumura, H. Shiozaki, M. Suzuki, I. Kurose, M. Suematsu, M. Tsuchiya, H. Ishii

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Changes in energy metabolism elicited by sodium taurocholate and their relation to cell viability were determined in gastric mucosal cells. Cultured mucosal cells were labelled with rhodamine-123, a mitochondrial energization-sensitive fluorescence probe, or by propidium iodide, a fluorochrome which labels the nuclei of non-viable cells. The cells were observed under a fluorescence microscope with a laser scanning confocal imaging system. After the addition of sodium taurocholate at concentrations >5 mol/L, mucosal cells showed a rapid and significant decrease in rhodamine-123 fluorescence. A decrease to 40% of the pretreated values at 30 min was seen with a concentration of sodium taurocholate of 7.5 mmol/L. A marked increase in the percentage of propidium iodide-positive cells was noted when the concentration of sodium taurocholate exceeded 5 mmol/L. However, the extent of the decrease in rhodamine-123 fluorescence was always greater than the increase in the percentage of propidium iodide-positive cells, suggesting that most of these gastric mucosal cells remained viable. It is therefore suggested that the decrease in rhodamine-123 fluorescence is largely due to the disturbed oxidative phosphorylation of mitochondria. Pretreatment of gastric mucosal cells with low concentrations of ethanol resulted in a significant cytoprotective effect against sodium taurocholate injury with significant prevention of a decrease in rhodamine-123 fluoresence. It is concluded that sodium taurocholate induces a depolarization of the mitochondrial membrane potential preceding cell injury and that the cytoprotective effect of ethanol relates to its attenuation of the uncoupling effect.

Original languageEnglish
Pages (from-to)621-626
Number of pages6
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume10
Issue number6
Publication statusPublished - 1995

Fingerprint

Mitochondrial Membrane Potential
Bile Acids and Salts
Pancreas
Stomach
Taurocholic Acid
Rhodamine 123
Wounds and Injuries
Fluorescence
Propidium
Ethanol
Oxidative Phosphorylation
Fluorescent Dyes
Energy Metabolism
Cultured Cells
Cell Survival
Mitochondria
Lasers

Keywords

  • Adaptive cytoprotection
  • Bile acid
  • Cell death
  • Gastric cell culture
  • Laser scanning confocal imaging system
  • Mitochondria
  • Propidium iodide
  • Rhodamine 123

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Alimentary tract and pancreas. Bile acid-induced depolarization of mitochondrial membrane potential preceding cell injury in cultured gastric mucosal cells. / Miura, S.; Fukumura, D.; Shiozaki, H.; Suzuki, M.; Kurose, I.; Suematsu, M.; Tsuchiya, M.; Ishii, H.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 10, No. 6, 1995, p. 621-626.

Research output: Contribution to journalArticle

Miura, S. ; Fukumura, D. ; Shiozaki, H. ; Suzuki, M. ; Kurose, I. ; Suematsu, M. ; Tsuchiya, M. ; Ishii, H. / Alimentary tract and pancreas. Bile acid-induced depolarization of mitochondrial membrane potential preceding cell injury in cultured gastric mucosal cells. In: Journal of Gastroenterology and Hepatology (Australia). 1995 ; Vol. 10, No. 6. pp. 621-626.
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AU - Suzuki, M.

AU - Kurose, I.

AU - Suematsu, M.

AU - Tsuchiya, M.

AU - Ishii, H.

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