Abstract
The present study was aimed to determine the allelopathic potential of mango (Mangifera indica L.) leaves and to identify allelopathic substances in the leaves. The aqueous methanol extracts of mango leaves inhibited seedling growth of garden cress, radish, rapeseed, foxtail fescue and crabgrass, and the inhibitory effects increased with the increasing extract concentration, suggesting that mango leaves may contain allelopathic substances. The extract was then purified by several chromatographic runs through a bioassay-directed fractionation, and an growth inhibitory substance was isolated and identified by spectral data as methyl-3,4,5-trihydroxybenzoate (methyl gallate). Methyl gallate at the concentration of 10 mM inhibited 98.6% and 99.8% of root and shoot growth of garden cress relative to the control, respectively, and 94.4% and 49.3% of those of foxtail fescue, respectively. The concentrations of methyl gallate required for 50% growth inhibition (I50) on garden cress roots and shoots were 3.9 and 3.3 mM, and those on foxtail fescue roots and shoots were 1.5 and 9.5 mM, respectively. It is the first report of an allelopathic substance in mango leaves and allelopathic activity of methyl gallate. Therefore, the mango leaves may have potential as a soil additive material for the weed management options.
| Original language | English |
|---|---|
| Pages (from-to) | 1-6 |
| Number of pages | 6 |
| Journal | Acta Agriculturae Scandinavica - Section B Soil and Plant Science |
| DOIs | |
| Publication status | Accepted/In press - 2016 Aug 4 |
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Keywords
- allelopathic substance
- Allelopathy
- growth inhibition
- mango
- methyl gallate
ASJC Scopus subject areas
- Agronomy and Crop Science
- Soil Science
Cite this
Allelopathic potential and an allelopathic substance in mango leaves. / Suzuki, Masahiko; Khan, Md Sirajul Islam; Iwasaki, Arihiro; Suenaga, Kiyotake; Kato-Noguchi, Hisashi.
In: Acta Agriculturae Scandinavica - Section B Soil and Plant Science, 04.08.2016, p. 1-6.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Allelopathic potential and an allelopathic substance in mango leaves
AU - Suzuki, Masahiko
AU - Khan, Md Sirajul Islam
AU - Iwasaki, Arihiro
AU - Suenaga, Kiyotake
AU - Kato-Noguchi, Hisashi
PY - 2016/8/4
Y1 - 2016/8/4
N2 - The present study was aimed to determine the allelopathic potential of mango (Mangifera indica L.) leaves and to identify allelopathic substances in the leaves. The aqueous methanol extracts of mango leaves inhibited seedling growth of garden cress, radish, rapeseed, foxtail fescue and crabgrass, and the inhibitory effects increased with the increasing extract concentration, suggesting that mango leaves may contain allelopathic substances. The extract was then purified by several chromatographic runs through a bioassay-directed fractionation, and an growth inhibitory substance was isolated and identified by spectral data as methyl-3,4,5-trihydroxybenzoate (methyl gallate). Methyl gallate at the concentration of 10 mM inhibited 98.6% and 99.8% of root and shoot growth of garden cress relative to the control, respectively, and 94.4% and 49.3% of those of foxtail fescue, respectively. The concentrations of methyl gallate required for 50% growth inhibition (I50) on garden cress roots and shoots were 3.9 and 3.3 mM, and those on foxtail fescue roots and shoots were 1.5 and 9.5 mM, respectively. It is the first report of an allelopathic substance in mango leaves and allelopathic activity of methyl gallate. Therefore, the mango leaves may have potential as a soil additive material for the weed management options.
AB - The present study was aimed to determine the allelopathic potential of mango (Mangifera indica L.) leaves and to identify allelopathic substances in the leaves. The aqueous methanol extracts of mango leaves inhibited seedling growth of garden cress, radish, rapeseed, foxtail fescue and crabgrass, and the inhibitory effects increased with the increasing extract concentration, suggesting that mango leaves may contain allelopathic substances. The extract was then purified by several chromatographic runs through a bioassay-directed fractionation, and an growth inhibitory substance was isolated and identified by spectral data as methyl-3,4,5-trihydroxybenzoate (methyl gallate). Methyl gallate at the concentration of 10 mM inhibited 98.6% and 99.8% of root and shoot growth of garden cress relative to the control, respectively, and 94.4% and 49.3% of those of foxtail fescue, respectively. The concentrations of methyl gallate required for 50% growth inhibition (I50) on garden cress roots and shoots were 3.9 and 3.3 mM, and those on foxtail fescue roots and shoots were 1.5 and 9.5 mM, respectively. It is the first report of an allelopathic substance in mango leaves and allelopathic activity of methyl gallate. Therefore, the mango leaves may have potential as a soil additive material for the weed management options.
KW - allelopathic substance
KW - Allelopathy
KW - growth inhibition
KW - mango
KW - methyl gallate
UR - http://www.scopus.com/inward/record.url?scp=84982822048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84982822048&partnerID=8YFLogxK
U2 - 10.1080/09064710.2016.1215517
DO - 10.1080/09064710.2016.1215517
M3 - Article
AN - SCOPUS:84982822048
SP - 1
EP - 6
JO - Acta Agriculturae Scandinavica - Section B Soil and Plant Science
JF - Acta Agriculturae Scandinavica - Section B Soil and Plant Science
SN - 0906-4710
ER -