Allelopathy of red pine: Isolation and identification of an allelopathic substance in red pine needles

Hisashi Kato-Noguchi, Yoshiko Fushimi, Yukitoshi Tanaka, Toshiaki Teruya, Kiyotake Suenaga

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Since red pine (Pinus densiflora Sieb. et Zucc.) often forms sparse forest floors where herbaceous plants do not grow well, allelopathy of red pine was investigated. A growth inhibitory substance was isolated from an aqueous methanol extract of red pine needles and determined by spectral data as abscisic acid-β-d-glucopyranosyl ester (ABA-GE). This substance inhibited root and shoot growth of cress and E. crus-galli seedlings at concentrations greater than 0. 1 μM. The concentrations required for 50% growth inhibition on roots and shoots of cress were 0.23 and 0.61 μM, respectively, and those of E. crus-galli were 1. 1 and 2. 8 μM, respectively. The activity of ABA-β-d-glucosidase, which liberates free ABA from ABA-GE, in cress and E. crus-galli seedlings was 13-29 nmol mg-1 protein min-1. Endogenous concentration of ABA-GE in the pine needles was 4. 1-21. 5 μmol kg-1 and the concentration in soil water of the pine forest was 2. 5 μM. The effectiveness of ABA-GE on growth inhibition and the occurrence of ABA-GE in pine needles and soil water suggest ABA-GE may play an important role in the allelopathy of red pine resulting in the formation of sparse forest floors.

Original languageEnglish
Pages (from-to)299-304
Number of pages6
JournalPlant Growth Regulation
Volume65
Issue number2
DOIs
Publication statusPublished - 2011 Nov 1

Keywords

  • Allelopathy
  • Growth inhibitor
  • Phytoalexin
  • Pinaceae
  • Pine forest

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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