Allotype analysis to determine the origin of cytomegalovirus immunoglobulin-G after allogeneic stem cell transplantation

R. Yamazaki, Y. Tanaka, H. Nakasone, M. Sato, K. Terasako-Saito, K. Sakamoto, Y. Akahoshi, H. Nakano, T. Ugai, R. Yamasaki, H. Wada, Y. Ishihara, K. Kawamura, M. Ashizawa, S. I. Kimura, M. Kikuchi, S. Kako, J. Kanda, A. Tanihara, J. NishidaY. Kanda

Research output: Contribution to journalArticle

1 Citation (Scopus)


Background: Cytomegalovirus (CMV) reactivation still remains a major problem following allogeneic hematopoietic stem cell transplantation (HSCT). Patients and methods: In this study, we analyzed an immunoglobulin allotype, IgG1m(f), in CMV-seropositive HSCT recipients and their donors to distinguish donor-derived antibody from recipient-derived antibody. Eight donor-recipient pairs were informative regarding the appearance of donor-derived immunoglobulin-G (IgG), as the recipients were homozygous null for the IgG1m(f) allotype and the donors were IgG1m(f) positive. In these patients, total IgG, IgM, and allotype-specific IgG against CMV were measured by enzyme-linked immunosorbent assay. All subjects were monitored for at least 9 months after HSCT with (n = 5) or without (n = 3) CMV reactivation. Results: Donor-derived CMV IgG tended to be elevated earlier in patients with CMV-seropositive donors than in those with CMV-seronegative donors. In 1 patient with a CMV-negative donor, donor-derived CMV IgG was not detected until late CMV reactivation. In 3 patients without CMV reactivation, donor-derived CMV IgG was also elevated within 1-6 months after HSCT. Conclusion: In conclusion, the CMV serostatus of the donor may be related to the timing of the appearance of donor-derived CMV IgG and the reconstitution of humoral immunity against CMV, regardless of the CMV antigenemia level after HSCT.

Original languageEnglish
Pages (from-to)904-913
Number of pages10
JournalTransplant Infectious Disease
Issue number6
Publication statusPublished - 2014 Dec 1
Externally publishedYes



  • Cytomegalovirus
  • Hematopoietic stem cell transplantation
  • Humoral immunity
  • Immunoglobulin allotypes

ASJC Scopus subject areas

  • Infectious Diseases
  • Transplantation

Cite this

Yamazaki, R., Tanaka, Y., Nakasone, H., Sato, M., Terasako-Saito, K., Sakamoto, K., Akahoshi, Y., Nakano, H., Ugai, T., Yamasaki, R., Wada, H., Ishihara, Y., Kawamura, K., Ashizawa, M., Kimura, S. I., Kikuchi, M., Kako, S., Kanda, J., Tanihara, A., ... Kanda, Y. (2014). Allotype analysis to determine the origin of cytomegalovirus immunoglobulin-G after allogeneic stem cell transplantation. Transplant Infectious Disease, 16(6), 904-913.