Allovahlkampfia spelaea Causing Keratitis in Humans

Mohammed Essa Marghany Tolba, Enas Abdelhameed Mahmoud Huseein, Haiam Mohamed Mahmoud Farrag, Hanan El Deek Mohamed, Seiki Kobayashi, Jun Suzuki, Tarek Ahmed Mohamed Ali, Sumio Sugano

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of family Vahlkampfiidae are rarely reported to cause such affections. A new genus, Allovahlkampfia spelaea was recently identified from caves with no data about pathogenicity in humans. We tried to identify the causative free-living amoeba in a case of keratitis in an Egyptian patient using morphological and molecular techniques. Methods: Pathogenic amoebae were culture using monoxenic culture system. Identification through morphological features and 18S ribosomal RNA subunit DNA amplification and sequencing was done. Pathogenicity to laboratory rabbits and ability to produce keratitis were assessed experimentally. Results: Allovahlkampfia spelaea was identified as a cause of human keratitis. Whole sequence of 18S ribosomal subunit DNA was sequenced and assembled. The Egyptian strain was closely related to SK1 strain isolated in Slovenia. The ability to induce keratitis was confirmed using animal model. Conclusions: This the first time to report Allovahlkampfia spelaea as a human pathogen. Combining both molecular and morphological identification is critical to correctly diagnose amoebae causing keratitis in humans. Use of different pairs of primers and sequencing amplified DNA is needed to prevent misdiagnosis.

Original languageEnglish
Article numbere0004841
JournalPLoS Neglected Tropical Diseases
Volume10
Issue number7
DOIs
Publication statusPublished - 2016 Jul 14

Fingerprint

Keratitis
Amoeba
Ribosome Subunits
Aptitude
DNA Sequence Analysis
Virulence
18S Ribosomal RNA
Acanthamoeba
Slovenia
Ribosomal DNA
Diagnostic Errors
Developing Countries
Animal Models
Rabbits

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Tolba, M. E. M., Huseein, E. A. M., Farrag, H. M. M., Mohamed, H. E. D., Kobayashi, S., Suzuki, J., ... Sugano, S. (2016). Allovahlkampfia spelaea Causing Keratitis in Humans. PLoS Neglected Tropical Diseases, 10(7), [e0004841]. https://doi.org/10.1371/journal.pntd.0004841

Allovahlkampfia spelaea Causing Keratitis in Humans. / Tolba, Mohammed Essa Marghany; Huseein, Enas Abdelhameed Mahmoud; Farrag, Haiam Mohamed Mahmoud; Mohamed, Hanan El Deek; Kobayashi, Seiki; Suzuki, Jun; Ali, Tarek Ahmed Mohamed; Sugano, Sumio.

In: PLoS Neglected Tropical Diseases, Vol. 10, No. 7, e0004841, 14.07.2016.

Research output: Contribution to journalArticle

Tolba, MEM, Huseein, EAM, Farrag, HMM, Mohamed, HED, Kobayashi, S, Suzuki, J, Ali, TAM & Sugano, S 2016, 'Allovahlkampfia spelaea Causing Keratitis in Humans', PLoS Neglected Tropical Diseases, vol. 10, no. 7, e0004841. https://doi.org/10.1371/journal.pntd.0004841
Tolba MEM, Huseein EAM, Farrag HMM, Mohamed HED, Kobayashi S, Suzuki J et al. Allovahlkampfia spelaea Causing Keratitis in Humans. PLoS Neglected Tropical Diseases. 2016 Jul 14;10(7). e0004841. https://doi.org/10.1371/journal.pntd.0004841
Tolba, Mohammed Essa Marghany ; Huseein, Enas Abdelhameed Mahmoud ; Farrag, Haiam Mohamed Mahmoud ; Mohamed, Hanan El Deek ; Kobayashi, Seiki ; Suzuki, Jun ; Ali, Tarek Ahmed Mohamed ; Sugano, Sumio. / Allovahlkampfia spelaea Causing Keratitis in Humans. In: PLoS Neglected Tropical Diseases. 2016 ; Vol. 10, No. 7.
@article{c6ba6779665e41bbb028c6469dd860ea,
title = "Allovahlkampfia spelaea Causing Keratitis in Humans",
abstract = "Background: Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of family Vahlkampfiidae are rarely reported to cause such affections. A new genus, Allovahlkampfia spelaea was recently identified from caves with no data about pathogenicity in humans. We tried to identify the causative free-living amoeba in a case of keratitis in an Egyptian patient using morphological and molecular techniques. Methods: Pathogenic amoebae were culture using monoxenic culture system. Identification through morphological features and 18S ribosomal RNA subunit DNA amplification and sequencing was done. Pathogenicity to laboratory rabbits and ability to produce keratitis were assessed experimentally. Results: Allovahlkampfia spelaea was identified as a cause of human keratitis. Whole sequence of 18S ribosomal subunit DNA was sequenced and assembled. The Egyptian strain was closely related to SK1 strain isolated in Slovenia. The ability to induce keratitis was confirmed using animal model. Conclusions: This the first time to report Allovahlkampfia spelaea as a human pathogen. Combining both molecular and morphological identification is critical to correctly diagnose amoebae causing keratitis in humans. Use of different pairs of primers and sequencing amplified DNA is needed to prevent misdiagnosis.",
author = "Tolba, {Mohammed Essa Marghany} and Huseein, {Enas Abdelhameed Mahmoud} and Farrag, {Haiam Mohamed Mahmoud} and Mohamed, {Hanan El Deek} and Seiki Kobayashi and Jun Suzuki and Ali, {Tarek Ahmed Mohamed} and Sumio Sugano",
year = "2016",
month = "7",
day = "14",
doi = "10.1371/journal.pntd.0004841",
language = "English",
volume = "10",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2727",
publisher = "Public Library of Science",
number = "7",

}

TY - JOUR

T1 - Allovahlkampfia spelaea Causing Keratitis in Humans

AU - Tolba, Mohammed Essa Marghany

AU - Huseein, Enas Abdelhameed Mahmoud

AU - Farrag, Haiam Mohamed Mahmoud

AU - Mohamed, Hanan El Deek

AU - Kobayashi, Seiki

AU - Suzuki, Jun

AU - Ali, Tarek Ahmed Mohamed

AU - Sugano, Sumio

PY - 2016/7/14

Y1 - 2016/7/14

N2 - Background: Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of family Vahlkampfiidae are rarely reported to cause such affections. A new genus, Allovahlkampfia spelaea was recently identified from caves with no data about pathogenicity in humans. We tried to identify the causative free-living amoeba in a case of keratitis in an Egyptian patient using morphological and molecular techniques. Methods: Pathogenic amoebae were culture using monoxenic culture system. Identification through morphological features and 18S ribosomal RNA subunit DNA amplification and sequencing was done. Pathogenicity to laboratory rabbits and ability to produce keratitis were assessed experimentally. Results: Allovahlkampfia spelaea was identified as a cause of human keratitis. Whole sequence of 18S ribosomal subunit DNA was sequenced and assembled. The Egyptian strain was closely related to SK1 strain isolated in Slovenia. The ability to induce keratitis was confirmed using animal model. Conclusions: This the first time to report Allovahlkampfia spelaea as a human pathogen. Combining both molecular and morphological identification is critical to correctly diagnose amoebae causing keratitis in humans. Use of different pairs of primers and sequencing amplified DNA is needed to prevent misdiagnosis.

AB - Background: Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of family Vahlkampfiidae are rarely reported to cause such affections. A new genus, Allovahlkampfia spelaea was recently identified from caves with no data about pathogenicity in humans. We tried to identify the causative free-living amoeba in a case of keratitis in an Egyptian patient using morphological and molecular techniques. Methods: Pathogenic amoebae were culture using monoxenic culture system. Identification through morphological features and 18S ribosomal RNA subunit DNA amplification and sequencing was done. Pathogenicity to laboratory rabbits and ability to produce keratitis were assessed experimentally. Results: Allovahlkampfia spelaea was identified as a cause of human keratitis. Whole sequence of 18S ribosomal subunit DNA was sequenced and assembled. The Egyptian strain was closely related to SK1 strain isolated in Slovenia. The ability to induce keratitis was confirmed using animal model. Conclusions: This the first time to report Allovahlkampfia spelaea as a human pathogen. Combining both molecular and morphological identification is critical to correctly diagnose amoebae causing keratitis in humans. Use of different pairs of primers and sequencing amplified DNA is needed to prevent misdiagnosis.

UR - http://www.scopus.com/inward/record.url?scp=84980329462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84980329462&partnerID=8YFLogxK

U2 - 10.1371/journal.pntd.0004841

DO - 10.1371/journal.pntd.0004841

M3 - Article

AN - SCOPUS:84980329462

VL - 10

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2727

IS - 7

M1 - e0004841

ER -