Alpha-ketoglutarate oxidoreductase, an essential salvage enzyme of energy metabolism, in coccoid form of Helicobacter pylori

Hitoshi Tsugawa, Hidekazu Suzuki, Izumi Nakagawa, Toshihiro Nishizawa, Yoshimasa Saito, Makoto Suematsu, Toshifumi Hibi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the Krebs cycle of Helicobacter pylori, the absence of alpha-ketoglutarate dehydrogenase and succinyl CoA synthetase are shown. Instead, alpha-ketoglutarate is converted to succinyl CoA and succinate by alpha-ketoglutarate oxidoreductase (KOR) and CoA transferase (CoAT). In the present study, when H. pylori transformed to the coccoid form, a viable but non-culturable form of H. pylori with reduced metabolic activity, the KOR activity was enhanced while the CoAT activity was reduced. Direct inactivation of KOR could potently kill the bacteria without allowing conversion to the coccoid form, suggesting a novel treatment strategy for the eradication of H. pylori, especially in cases infected with multiple antibiotic-resistant strains.

Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume376
Issue number1
DOIs
Publication statusPublished - 2008 Nov 7

Keywords

  • Alpha-ketoglutarate oxidoreductase
  • CoA transferase
  • Coccoid form
  • Helicobacter pylori
  • Krebs cycle

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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