Alteration in the contents of unsaturated fatty acids in dnaA mutants of Escherichia coli

Emi Suzuki, Taemi Kondo, Masaki Makise, Shinji Mima, Kenji Sakamoto, Tomofusa Tsuchiya, Tohru Mizushima

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Abstract

DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli, has a high affinity for acidic phospholipids containing unsaturated fatty acids. We have examined here the fatty acid composition of phospholipids in dnaA mutants. A temperature sensitive dnaA46 mutant showed a lower level of unsaturation of fatty acids (ratio of unsaturated to saturated fatty acids) at 42°C (non-permissive temperature) and at 37°C (semi-permissive temperature), but not at 28°C (permissive temperature), compared with the wild-type strain. Plasmid complementation analysis revealed that the dnaA46 mutation is responsible for the phenotype. Other temperature-sensitive dnaA mutants showed similar results. On the other hand, a cold-sensitive dnaAcos mutant, in which overinitiation of DNA replication occurs at low temperature (28°C), showed a higher level of unsaturation of fatty acids at 28°C. Based on these observations, we discuss the role of phospholipids in the regulation of the activity of DnaA protein.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalMolecular Microbiology
Volume28
Issue number1
DOIs
Publication statusPublished - 1998 Apr 21

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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    Suzuki, E., Kondo, T., Makise, M., Mima, S., Sakamoto, K., Tsuchiya, T., & Mizushima, T. (1998). Alteration in the contents of unsaturated fatty acids in dnaA mutants of Escherichia coli. Molecular Microbiology, 28(1), 95-102. https://doi.org/10.1046/j.1365-2958.1998.00777.x