Alteration of asparagine-linked glycosylation in serum transferrin of patients with hepatocellular carcinoma

Kojiro Matsumoto, Yoshie Maeda, Shinzo Kato, Hidetaka Yuki

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The asparagine-linked sugar chains in serum transferrin purified from patients with hepatocellular carcinoma (n = 13), healthy individuals (n = 5) and patients with liver cirrhosis (n = 6) were compared. Sugar chains released with N-glycanase from desialylated and pepsin-digested transferrin were derivatized by reductive pyridylamination. Analysis of the sugar chains by high performance liquid chromatography in combination with exoglycosidase digestion revealed an increase of a biantennary complex-type sugar chain with a fucosylated trimannosyl core; Galβ1-4GlcNAcβ1-2Manαl-6(Galβ1-4GlcNAcβ1-2Manαl-3) Manβ1-4GlcNAcβ1-4(Fucαl-6)GlcNAc in 7 of 13 cancer patients and an increase of a sugar chain with a fucosylated trimannosyl core and bisecting N-acetylglucosamine; Galβ 1-4GlcNAc-β1-2Manαl-6(GlcNAcβ1-4) (Galβ1-4GlcNAcβ1-2Manαl-3)Manβ1-4GlcNAcβ1-4(Fuc-α 1-6)GlcNAc in one of the 13 cancer patients. Further, the fucosylated alteration of the sugar chain was detected also in α1-antitrypsin, hemopexin, α1-acid glycoprotein and α2-HS glycoprotein from one of the patients with increased fucosylated transferrin.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalClinica Chimica Acta
Volume224
Issue number1
DOIs
Publication statusPublished - 1994 Jan 14

Keywords

  • Fucosylation
  • Hepatocellular carcinoma
  • Oligosaccharides
  • Transferrin

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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