Alteration of asparagine-linked glycosylation in serum transferrin of patients with hepatocellular carcinoma

Kojiro Matsumoto, Yoshie Maeda, Shinzo Kato, Hidetaka Yuki

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The asparagine-linked sugar chains in serum transferrin purified from patients with hepatocellular carcinoma (n = 13), healthy individuals (n = 5) and patients with liver cirrhosis (n = 6) were compared. Sugar chains released with N-glycanase from desialylated and pepsin-digested transferrin were derivatized by reductive pyridylamination. Analysis of the sugar chains by high performance liquid chromatography in combination with exoglycosidase digestion revealed an increase of a biantennary complex-type sugar chain with a fucosylated trimannosyl core; Galβ1-4GlcNAcβ1-2Manαl-6(Galβ1-4GlcNAcβ1-2Manαl-3) Manβ1-4GlcNAcβ1-4(Fucαl-6)GlcNAc in 7 of 13 cancer patients and an increase of a sugar chain with a fucosylated trimannosyl core and bisecting N-acetylglucosamine; Galβ 1-4GlcNAc-β1-2Manαl-6(GlcNAcβ1-4) (Galβ1-4GlcNAcβ1-2Manαl-3)Manβ1-4GlcNAcβ1-4(Fuc-α 1-6)GlcNAc in one of the 13 cancer patients. Further, the fucosylated alteration of the sugar chain was detected also in α1-antitrypsin, hemopexin, α1-acid glycoprotein and α2-HS glycoprotein from one of the patients with increased fucosylated transferrin.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalClinica Chimica Acta
Volume224
Issue number1
DOIs
Publication statusPublished - 1994 Jan 14

Fingerprint

Glycosylation
Asparagine
Transferrin
Sugars
Hepatocellular Carcinoma
Serum
Glycoproteins
Hemopexin
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Acetylglucosamine
Glycoside Hydrolases
Pepsin A
Liver Cirrhosis
High performance liquid chromatography
Digestion
Neoplasms
Liver
High Pressure Liquid Chromatography
Acids

Keywords

  • Fucosylation
  • Hepatocellular carcinoma
  • Oligosaccharides
  • Transferrin

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Alteration of asparagine-linked glycosylation in serum transferrin of patients with hepatocellular carcinoma. / Matsumoto, Kojiro; Maeda, Yoshie; Kato, Shinzo; Yuki, Hidetaka.

In: Clinica Chimica Acta, Vol. 224, No. 1, 14.01.1994, p. 1-8.

Research output: Contribution to journalArticle

@article{70ba6799f7fd4f4a8821a97b58ab30a0,
title = "Alteration of asparagine-linked glycosylation in serum transferrin of patients with hepatocellular carcinoma",
abstract = "The asparagine-linked sugar chains in serum transferrin purified from patients with hepatocellular carcinoma (n = 13), healthy individuals (n = 5) and patients with liver cirrhosis (n = 6) were compared. Sugar chains released with N-glycanase from desialylated and pepsin-digested transferrin were derivatized by reductive pyridylamination. Analysis of the sugar chains by high performance liquid chromatography in combination with exoglycosidase digestion revealed an increase of a biantennary complex-type sugar chain with a fucosylated trimannosyl core; Galβ1-4GlcNAcβ1-2Manαl-6(Galβ1-4GlcNAcβ1-2Manαl-3) Manβ1-4GlcNAcβ1-4(Fucαl-6)GlcNAc in 7 of 13 cancer patients and an increase of a sugar chain with a fucosylated trimannosyl core and bisecting N-acetylglucosamine; Galβ 1-4GlcNAc-β1-2Manαl-6(GlcNAcβ1-4) (Galβ1-4GlcNAcβ1-2Manαl-3)Manβ1-4GlcNAcβ1-4(Fuc-α 1-6)GlcNAc in one of the 13 cancer patients. Further, the fucosylated alteration of the sugar chain was detected also in α1-antitrypsin, hemopexin, α1-acid glycoprotein and α2-HS glycoprotein from one of the patients with increased fucosylated transferrin.",
keywords = "Fucosylation, Hepatocellular carcinoma, Oligosaccharides, Transferrin",
author = "Kojiro Matsumoto and Yoshie Maeda and Shinzo Kato and Hidetaka Yuki",
year = "1994",
month = "1",
day = "14",
doi = "10.1016/0009-8981(94)90115-5",
language = "English",
volume = "224",
pages = "1--8",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Alteration of asparagine-linked glycosylation in serum transferrin of patients with hepatocellular carcinoma

AU - Matsumoto, Kojiro

AU - Maeda, Yoshie

AU - Kato, Shinzo

AU - Yuki, Hidetaka

PY - 1994/1/14

Y1 - 1994/1/14

N2 - The asparagine-linked sugar chains in serum transferrin purified from patients with hepatocellular carcinoma (n = 13), healthy individuals (n = 5) and patients with liver cirrhosis (n = 6) were compared. Sugar chains released with N-glycanase from desialylated and pepsin-digested transferrin were derivatized by reductive pyridylamination. Analysis of the sugar chains by high performance liquid chromatography in combination with exoglycosidase digestion revealed an increase of a biantennary complex-type sugar chain with a fucosylated trimannosyl core; Galβ1-4GlcNAcβ1-2Manαl-6(Galβ1-4GlcNAcβ1-2Manαl-3) Manβ1-4GlcNAcβ1-4(Fucαl-6)GlcNAc in 7 of 13 cancer patients and an increase of a sugar chain with a fucosylated trimannosyl core and bisecting N-acetylglucosamine; Galβ 1-4GlcNAc-β1-2Manαl-6(GlcNAcβ1-4) (Galβ1-4GlcNAcβ1-2Manαl-3)Manβ1-4GlcNAcβ1-4(Fuc-α 1-6)GlcNAc in one of the 13 cancer patients. Further, the fucosylated alteration of the sugar chain was detected also in α1-antitrypsin, hemopexin, α1-acid glycoprotein and α2-HS glycoprotein from one of the patients with increased fucosylated transferrin.

AB - The asparagine-linked sugar chains in serum transferrin purified from patients with hepatocellular carcinoma (n = 13), healthy individuals (n = 5) and patients with liver cirrhosis (n = 6) were compared. Sugar chains released with N-glycanase from desialylated and pepsin-digested transferrin were derivatized by reductive pyridylamination. Analysis of the sugar chains by high performance liquid chromatography in combination with exoglycosidase digestion revealed an increase of a biantennary complex-type sugar chain with a fucosylated trimannosyl core; Galβ1-4GlcNAcβ1-2Manαl-6(Galβ1-4GlcNAcβ1-2Manαl-3) Manβ1-4GlcNAcβ1-4(Fucαl-6)GlcNAc in 7 of 13 cancer patients and an increase of a sugar chain with a fucosylated trimannosyl core and bisecting N-acetylglucosamine; Galβ 1-4GlcNAc-β1-2Manαl-6(GlcNAcβ1-4) (Galβ1-4GlcNAcβ1-2Manαl-3)Manβ1-4GlcNAcβ1-4(Fuc-α 1-6)GlcNAc in one of the 13 cancer patients. Further, the fucosylated alteration of the sugar chain was detected also in α1-antitrypsin, hemopexin, α1-acid glycoprotein and α2-HS glycoprotein from one of the patients with increased fucosylated transferrin.

KW - Fucosylation

KW - Hepatocellular carcinoma

KW - Oligosaccharides

KW - Transferrin

UR - http://www.scopus.com/inward/record.url?scp=0028296132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028296132&partnerID=8YFLogxK

U2 - 10.1016/0009-8981(94)90115-5

DO - 10.1016/0009-8981(94)90115-5

M3 - Article

C2 - 8174273

AN - SCOPUS:0028296132

VL - 224

SP - 1

EP - 8

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1

ER -