Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB

K. Tanaka, Y. Fukuuchi, S. Nogawa, H. Nozaki, E. Nagata, Shigeaki Suzuki, T. Dembo, A. Kosakai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Binding of cAMP to the regulatory subunit of cAMP-dependent protein kinase (PKA) is an essential step for cAMP-mediated signal transduction including phosphorylation of cAMP response element binding protein (CREB). In the present study, binding activity of PKA with cAMP and CREB phosphorylation were examined in rat focal brain ischemia induced by occlusion of the middle cerebral artery for 1.5 hours followed by various time of recirculation. Binding activity of PKA with cAMP was progressively inhibited during the acute phase of ischemia from the ischemic core to peri-ischemia area. Phosphorylated CREB-positive cells in the ischemic core revealed a significant, but transient increase in number at 3.5 hours of recirculation, followed by a rapid decrease below the control level during the subsequent period. On the other hand, in the peri-ischemia area, the number of phosphorylated CREB-positive cells showed a more marked increase as compared to that in the ischemic core, and the increase continued until 48 hours of recirculation with a tendency for gradual decline. Persistent enhancement of CREB phosphorylation may thus be closely related to the neuronal viability and neuroprotective mechanisms, whereas rapid disappearance of CREB phosphorylation following ischemic insult may clearly precede neuronal death.

Original languageEnglish
Pages (from-to)1298-1299
Number of pages2
JournalClinical Neurology
Volume39
Issue number12
Publication statusPublished - 1999 Dec

Fingerprint

Cyclic AMP Response Element-Binding Protein
Brain Ischemia
Protein Kinases
Signal Transduction
Carrier Proteins
Phosphorylation
Ischemia
Middle Cerebral Artery Infarction
Cyclic AMP-Dependent Protein Kinases

Keywords

  • Cerebral ischemia
  • CREB
  • Cyclic AMP
  • Signal transduction

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Tanaka, K., Fukuuchi, Y., Nogawa, S., Nozaki, H., Nagata, E., Suzuki, S., ... Kosakai, A. (1999). Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB. Clinical Neurology, 39(12), 1298-1299.

Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB. / Tanaka, K.; Fukuuchi, Y.; Nogawa, S.; Nozaki, H.; Nagata, E.; Suzuki, Shigeaki; Dembo, T.; Kosakai, A.

In: Clinical Neurology, Vol. 39, No. 12, 12.1999, p. 1298-1299.

Research output: Contribution to journalArticle

Tanaka, K, Fukuuchi, Y, Nogawa, S, Nozaki, H, Nagata, E, Suzuki, S, Dembo, T & Kosakai, A 1999, 'Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB', Clinical Neurology, vol. 39, no. 12, pp. 1298-1299.
Tanaka, K. ; Fukuuchi, Y. ; Nogawa, S. ; Nozaki, H. ; Nagata, E. ; Suzuki, Shigeaki ; Dembo, T. ; Kosakai, A. / Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB. In: Clinical Neurology. 1999 ; Vol. 39, No. 12. pp. 1298-1299.
@article{6cdb3020543841aa8762a9941586087e,
title = "Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB",
abstract = "Binding of cAMP to the regulatory subunit of cAMP-dependent protein kinase (PKA) is an essential step for cAMP-mediated signal transduction including phosphorylation of cAMP response element binding protein (CREB). In the present study, binding activity of PKA with cAMP and CREB phosphorylation were examined in rat focal brain ischemia induced by occlusion of the middle cerebral artery for 1.5 hours followed by various time of recirculation. Binding activity of PKA with cAMP was progressively inhibited during the acute phase of ischemia from the ischemic core to peri-ischemia area. Phosphorylated CREB-positive cells in the ischemic core revealed a significant, but transient increase in number at 3.5 hours of recirculation, followed by a rapid decrease below the control level during the subsequent period. On the other hand, in the peri-ischemia area, the number of phosphorylated CREB-positive cells showed a more marked increase as compared to that in the ischemic core, and the increase continued until 48 hours of recirculation with a tendency for gradual decline. Persistent enhancement of CREB phosphorylation may thus be closely related to the neuronal viability and neuroprotective mechanisms, whereas rapid disappearance of CREB phosphorylation following ischemic insult may clearly precede neuronal death.",
keywords = "Cerebral ischemia, CREB, Cyclic AMP, Signal transduction",
author = "K. Tanaka and Y. Fukuuchi and S. Nogawa and H. Nozaki and E. Nagata and Shigeaki Suzuki and T. Dembo and A. Kosakai",
year = "1999",
month = "12",
language = "English",
volume = "39",
pages = "1298--1299",
journal = "Clinical Neurology",
issn = "0009-918X",
publisher = "Societas Neurologica Japonica",
number = "12",

}

TY - JOUR

T1 - Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB

AU - Tanaka, K.

AU - Fukuuchi, Y.

AU - Nogawa, S.

AU - Nozaki, H.

AU - Nagata, E.

AU - Suzuki, Shigeaki

AU - Dembo, T.

AU - Kosakai, A.

PY - 1999/12

Y1 - 1999/12

N2 - Binding of cAMP to the regulatory subunit of cAMP-dependent protein kinase (PKA) is an essential step for cAMP-mediated signal transduction including phosphorylation of cAMP response element binding protein (CREB). In the present study, binding activity of PKA with cAMP and CREB phosphorylation were examined in rat focal brain ischemia induced by occlusion of the middle cerebral artery for 1.5 hours followed by various time of recirculation. Binding activity of PKA with cAMP was progressively inhibited during the acute phase of ischemia from the ischemic core to peri-ischemia area. Phosphorylated CREB-positive cells in the ischemic core revealed a significant, but transient increase in number at 3.5 hours of recirculation, followed by a rapid decrease below the control level during the subsequent period. On the other hand, in the peri-ischemia area, the number of phosphorylated CREB-positive cells showed a more marked increase as compared to that in the ischemic core, and the increase continued until 48 hours of recirculation with a tendency for gradual decline. Persistent enhancement of CREB phosphorylation may thus be closely related to the neuronal viability and neuroprotective mechanisms, whereas rapid disappearance of CREB phosphorylation following ischemic insult may clearly precede neuronal death.

AB - Binding of cAMP to the regulatory subunit of cAMP-dependent protein kinase (PKA) is an essential step for cAMP-mediated signal transduction including phosphorylation of cAMP response element binding protein (CREB). In the present study, binding activity of PKA with cAMP and CREB phosphorylation were examined in rat focal brain ischemia induced by occlusion of the middle cerebral artery for 1.5 hours followed by various time of recirculation. Binding activity of PKA with cAMP was progressively inhibited during the acute phase of ischemia from the ischemic core to peri-ischemia area. Phosphorylated CREB-positive cells in the ischemic core revealed a significant, but transient increase in number at 3.5 hours of recirculation, followed by a rapid decrease below the control level during the subsequent period. On the other hand, in the peri-ischemia area, the number of phosphorylated CREB-positive cells showed a more marked increase as compared to that in the ischemic core, and the increase continued until 48 hours of recirculation with a tendency for gradual decline. Persistent enhancement of CREB phosphorylation may thus be closely related to the neuronal viability and neuroprotective mechanisms, whereas rapid disappearance of CREB phosphorylation following ischemic insult may clearly precede neuronal death.

KW - Cerebral ischemia

KW - CREB

KW - Cyclic AMP

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=0033502565&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033502565&partnerID=8YFLogxK

M3 - Article

C2 - 10791102

AN - SCOPUS:0033502565

VL - 39

SP - 1298

EP - 1299

JO - Clinical Neurology

JF - Clinical Neurology

SN - 0009-918X

IS - 12

ER -