Alteration of membrane-associated enzymes in drug-resistant sublines of mouse lymphoblastoma L5178Y cells

Yoshikazu Sugimoto, T. Nishimura, H. Suzuki, N. Tanaka

Research output: Contribution to journalArticle

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Abstract

Activities of marker enzymes for various cell components were studied with extracts of adriamycin-, aclacinomycin A- and bleomycin-resistant cells and with partially purified plasma membrane fraction of aclacinomycin A-resistant cells, in comparison with those of the parental cells. Alkaline phosphodiesterase and Na+-K+-ATPase activities were observed to alter in the drug-resistant sublines, but other enzymes showed similar activites in the resistant cells to those in the parental cells. Alkaline phosphodiesterase activities in all the resistant sublines were higher than that in the parental cells. Na+-K+-ATPase activities of anthracycline-resistant sublines were lower and that in bleomycin-resistant cell line was higher than that of the parental cells. The adriamycin-resistant cells exhibited the same level of alkaline phosphodiesterase activity with the aclacinomycin A-resistant cells: V(max) was the same with, and the affinity was twice stronger than the parental cells. The bleomycin-resistant cells showed ca. 30% V(max) in comparison with the sensitive cells, and 17 fold higher affinity than the parental cells. The current results, concerning changes of membrane-associated enzymes in drug-resistant sublines of L5178Y cells, support the assumption that the resistance is due to alteration of plasma membrane transport systems.

Original languageEnglish
Pages (from-to)1200-1205
Number of pages6
JournalJournal of Antibiotics
Volume34
Issue number9
Publication statusPublished - 1981
Externally publishedYes

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Membranes
Enzymes
Pharmaceutical Preparations
Aclarubicin
Phosphodiesterase I
Bleomycin
Doxorubicin
Cell Membrane
Anthracyclines
Cellular Structures
Cell Line

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Alteration of membrane-associated enzymes in drug-resistant sublines of mouse lymphoblastoma L5178Y cells. / Sugimoto, Yoshikazu; Nishimura, T.; Suzuki, H.; Tanaka, N.

In: Journal of Antibiotics, Vol. 34, No. 9, 1981, p. 1200-1205.

Research output: Contribution to journalArticle

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