Alteration of metabolomic profiles by titanium dioxide nanoparticles in human gingivitis model

Rene Garcia-Contreras, Masahiro Sugimoto, Naoki Umemura, Miku Kaneko, Yoko Hatakeyama, Tomoyoshi Soga, Masaru Tomita, Rogelio J. Scougall-Vilchis, Rosalia Contreras-Bulnes, Hiroshi Nakajima, Hiroshi Sakagami

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Although nanoparticles (NPs) has afforded considerable benefits in various fields of sciences, several reports have shown their harmful effects, suggesting the necessity of adequate risk assessment. To clarify the mechanism of titanium dioxide nanoparticles (TiO<inf>2</inf> NPs)-enhanced gingival inflammation, we conducted the full-scale metabolomic analyses of human gingival fibroblast cells treated with IL-1β alone or in combination with TiO<inf>2</inf> NPs. Observation with transmission electron microscope demonstrated the incorporation of TiO<inf>2</inf> NPs into vacuoles of the cells. TiO<inf>2</inf> NPs significantly enhanced the IL-1β-induced prostaglandin E<inf>2</inf> production and COX-1 and COX-2 protein expression. IL-1β reduced the intracellular concentrations of overall primary metabolites especially those of amino acid, urea cycle, polyamine, S-adenosylmethione and glutathione synthetic pathways. The addition of TiO<inf>2</inf> NPs further augmented these IL-1β-induced metabolic changes, recommending careful use of dental materials containing TiO<inf>2</inf> NPs towards patients with gingivitis or periodontitis. The impact of the present study is to identify the molecular targets of TiO<inf>2</inf> NPs for the future establishment of new metabolic markers and therapeutic strategy of gingival inflammation.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalBiomaterials
Volume57
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

Gingivitis
Metabolomics
Nanoparticles
Titanium dioxide
Interleukin-1
Dental materials
Inflammation
titanium dioxide
Dental Materials
Periodontitis
Polyamines
Fibroblasts
Metabolites
Vacuoles
Dinoprostone
Urea
Risk assessment
Glutathione
Amino acids
Electron microscopes

Keywords

  • Human gingival fibroblast
  • Inerleukin-1β
  • Inflammation
  • Metabolomics
  • Titanium dioxide nanoparticle

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Garcia-Contreras, R., Sugimoto, M., Umemura, N., Kaneko, M., Hatakeyama, Y., Soga, T., ... Sakagami, H. (2015). Alteration of metabolomic profiles by titanium dioxide nanoparticles in human gingivitis model. Biomaterials, 57, 33-40. https://doi.org/10.1016/j.biomaterials.2015.03.059

Alteration of metabolomic profiles by titanium dioxide nanoparticles in human gingivitis model. / Garcia-Contreras, Rene; Sugimoto, Masahiro; Umemura, Naoki; Kaneko, Miku; Hatakeyama, Yoko; Soga, Tomoyoshi; Tomita, Masaru; Scougall-Vilchis, Rogelio J.; Contreras-Bulnes, Rosalia; Nakajima, Hiroshi; Sakagami, Hiroshi.

In: Biomaterials, Vol. 57, 01.07.2015, p. 33-40.

Research output: Contribution to journalArticle

Garcia-Contreras, R, Sugimoto, M, Umemura, N, Kaneko, M, Hatakeyama, Y, Soga, T, Tomita, M, Scougall-Vilchis, RJ, Contreras-Bulnes, R, Nakajima, H & Sakagami, H 2015, 'Alteration of metabolomic profiles by titanium dioxide nanoparticles in human gingivitis model', Biomaterials, vol. 57, pp. 33-40. https://doi.org/10.1016/j.biomaterials.2015.03.059
Garcia-Contreras, Rene ; Sugimoto, Masahiro ; Umemura, Naoki ; Kaneko, Miku ; Hatakeyama, Yoko ; Soga, Tomoyoshi ; Tomita, Masaru ; Scougall-Vilchis, Rogelio J. ; Contreras-Bulnes, Rosalia ; Nakajima, Hiroshi ; Sakagami, Hiroshi. / Alteration of metabolomic profiles by titanium dioxide nanoparticles in human gingivitis model. In: Biomaterials. 2015 ; Vol. 57. pp. 33-40.
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