TY - JOUR
T1 - Alteration of plasma membrane of drug-resistant tumor cells
T2 - 230-Kilodalton protein identified by monoclonal antibody
AU - Sugimoto, Yoshikazu
AU - Suzuki, Hideo
AU - Tanaka, Nobuo
N1 - Funding Information:
ACKNOWLEDGEMENTS The current works were partly supported by a grant-in-aid cancer research from the Ministry of Education, Science and Culture, Japan. The authors express their deep thanks to Dr. H. Umezawa, Institute of Microbial Chemistry, for his kind advice and cooperation throughout the present experiments.
PY - 1983/8/12
Y1 - 1983/8/12
N2 - A number of hybridomas producing antibodies to plasma membrane of an aclarubicin-resistant subline of L5178Y cells were obtained. Among the hybridoma antibodies, one was found by agglutination tests to react with the aclarubicinresistant cell line, but not significantly with the parental and adriamycin-, bleomycin- and macromomycin-resistant cell lines. The monoclonal antibody was designated SC438, and showed complement-dependent cytolytic activity more markedly against the aclarubicin-resistant cells than against the parental cells. Fluorographs of [14C] leucine-labeled aclarubicin-resistant cells demonstrated two protein bands of 230k and 20k daltons, which were precipitated by the SC438 antibody. The former seemed to be specific for the aclarubicin-resistant cells.
AB - A number of hybridomas producing antibodies to plasma membrane of an aclarubicin-resistant subline of L5178Y cells were obtained. Among the hybridoma antibodies, one was found by agglutination tests to react with the aclarubicinresistant cell line, but not significantly with the parental and adriamycin-, bleomycin- and macromomycin-resistant cell lines. The monoclonal antibody was designated SC438, and showed complement-dependent cytolytic activity more markedly against the aclarubicin-resistant cells than against the parental cells. Fluorographs of [14C] leucine-labeled aclarubicin-resistant cells demonstrated two protein bands of 230k and 20k daltons, which were precipitated by the SC438 antibody. The former seemed to be specific for the aclarubicin-resistant cells.
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U2 - 10.1016/0006-291X(83)90655-1
DO - 10.1016/0006-291X(83)90655-1
M3 - Article
C2 - 6577861
AN - SCOPUS:0020578572
SN - 0006-291X
VL - 114
SP - 969
EP - 975
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -