Alterations in protein kinase C activity and membrane lipid metabolism in cerebral vasospasm after subarachnoid hemorrhage

Yoh Takuwa, Toru Matsui, Yoichiro Abe, Toshiaki Nagafuji, Kamejiro Yamashita, Takao Asano

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Summary: Changes in protein kinase C (PKC) activity, membrane lipid metabolism, and the extent of 20-kDa myosin light chain (MLC) phosphorylation in spastic cerebral basilar arteries were examined by using the beagle "two-hemorrhage" model of subarachnoid hemorrhage. In spastic arteries at days 4 and 7, cytosolic PKC activity showed a decrease of 40-45% with no significant changes in membrane PKC activity as compared with nonspastic control arteries. Cytosolic PKC activity of the day 14 arteries returned toward the normal control level with the remission of vasospasm. Western blot analysis of the PKC isoforms revealed that the amounts of PKCα and PKCε but not PKCζ were decreased in spastic arteries. As compared with nonspastic arteries, spastic arteries showed higher rates of incorporation of [3H]choline into phosphatidylcholine (PC) and [14C]ethanolamine into phosphatidylethanolamine (PE), but not of [3H]myoinositol into phosphoinositides, suggesting the stimulated turnover of PC and PE. The extent of 20-kDa MLC phosphorylation was not increased in the spastic arteries at days 4 or 7 as compared with that in the nonspastic control arteries. These results demonstrate that PKC activity and related membrane lipid metabolism are altered in spastic basilar arteries after subarachnoid hemorrhage.

Original languageEnglish
Pages (from-to)409-415
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume13
Issue number3
Publication statusPublished - 1993
Externally publishedYes

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Intracranial Vasospasm
Subarachnoid Hemorrhage
Membrane Lipids
Lipid Metabolism
Protein Kinase C
Muscle Spasticity
Arteries
Myosin Light Chains
Basilar Artery
Phosphatidylcholines
Phosphorylation
Ethanolamine
Cerebral Arteries
Inositol
Phosphatidylinositols
Choline
Protein Isoforms
Membrane Proteins
Western Blotting
Hemorrhage

Keywords

  • Cerebral vasospasm
  • Myosin light chain phosphorylation
  • Phosphatidylcholine turnover
  • Protein kinase c
  • Subarachnoid hemorrhage

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Neuroscience(all)

Cite this

Alterations in protein kinase C activity and membrane lipid metabolism in cerebral vasospasm after subarachnoid hemorrhage. / Takuwa, Yoh; Matsui, Toru; Abe, Yoichiro; Nagafuji, Toshiaki; Yamashita, Kamejiro; Asano, Takao.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 13, No. 3, 1993, p. 409-415.

Research output: Contribution to journalArticle

Takuwa, Yoh ; Matsui, Toru ; Abe, Yoichiro ; Nagafuji, Toshiaki ; Yamashita, Kamejiro ; Asano, Takao. / Alterations in protein kinase C activity and membrane lipid metabolism in cerebral vasospasm after subarachnoid hemorrhage. In: Journal of Cerebral Blood Flow and Metabolism. 1993 ; Vol. 13, No. 3. pp. 409-415.
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N2 - Summary: Changes in protein kinase C (PKC) activity, membrane lipid metabolism, and the extent of 20-kDa myosin light chain (MLC) phosphorylation in spastic cerebral basilar arteries were examined by using the beagle "two-hemorrhage" model of subarachnoid hemorrhage. In spastic arteries at days 4 and 7, cytosolic PKC activity showed a decrease of 40-45% with no significant changes in membrane PKC activity as compared with nonspastic control arteries. Cytosolic PKC activity of the day 14 arteries returned toward the normal control level with the remission of vasospasm. Western blot analysis of the PKC isoforms revealed that the amounts of PKCα and PKCε but not PKCζ were decreased in spastic arteries. As compared with nonspastic arteries, spastic arteries showed higher rates of incorporation of [3H]choline into phosphatidylcholine (PC) and [14C]ethanolamine into phosphatidylethanolamine (PE), but not of [3H]myoinositol into phosphoinositides, suggesting the stimulated turnover of PC and PE. The extent of 20-kDa MLC phosphorylation was not increased in the spastic arteries at days 4 or 7 as compared with that in the nonspastic control arteries. These results demonstrate that PKC activity and related membrane lipid metabolism are altered in spastic basilar arteries after subarachnoid hemorrhage.

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