Alterations in the glycolipid composition and cellular properties of ovarian carcinoma-derived RMG-1 cells on transfection of the α1,2-fucosyltransferase gene

Masao Iwamori, Kyoko Tanaka, Kaneyuki Kubushiro, Bei Lin, Kazushige Kiguchi, Isamu Ishiwata, Katsumi Tsukazaki, Shiro Nozawa

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Transfection of the mouse Fut1 and Fut2, and human FUT1 genes into human ovarian carcinoma-derived RMG-1 cells resulted in 20-30-fold increases in cellular α1,2-fucosyltransferase activity, and in alteration of the glycolipid composition, including not only fucosylated products, but also precursor glycolipids. Although globo-series glycolipids were not significantly affected by the transfection, the major glycolipids belonging to the lacto-series type 1 chain family in RMG-1 cells and the transfectants were the Lc4Cer, Lewis a (Le)a and Leb, and H-1 glycolipids, respectively, suggesting that fucosylation of Lc4Cer to the H-1 glycolipid prevents the further modification of Lc4Cer to Lea and Leb in the transfectants. Also, the lacto-series type 2 chains in RMG-1 cells were LeX, NeuAc-nLC4Cer and NeuAc-LeX, and those in the transfectants were LeX and LeY, indicating that the sialylation of nLc4Cer and LeX is restricted by increased fucosylation of LeX. As a result, the amount of sialic acid released by sialidase from the transfectants decreased to 70% of that from RMG-1 cells, and several membrane-mediated phenomena, such as the cell-to-cell interaction between cancer cells and mesothelial cells, and the cell viability in the presence of an anticancer drug, 5-fluorouracil, for the transfectants was found to be increased in comparison to that for RMG-1 cells. These findings indicate that cell surface carbohydrates are involved in the biological properties, including cell-to-cell adhesion and drug resistance, of cancer cells.

Original languageEnglish
Pages (from-to)26-30
Number of pages5
JournalCancer Science
Volume96
Issue number1
DOIs
Publication statusPublished - 2005 Jan

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Glycolipids
Transfection
Carcinoma
Genes
galactoside 2-alpha-L-fucosyltransferase
Neuraminidase
N-Acetylneuraminic Acid
Drug Resistance
Cell Adhesion
Cell Communication
Fluorouracil
Neoplasms
Cell Survival
Carbohydrates
Cell Membrane

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Alterations in the glycolipid composition and cellular properties of ovarian carcinoma-derived RMG-1 cells on transfection of the α1,2-fucosyltransferase gene. / Iwamori, Masao; Tanaka, Kyoko; Kubushiro, Kaneyuki; Lin, Bei; Kiguchi, Kazushige; Ishiwata, Isamu; Tsukazaki, Katsumi; Nozawa, Shiro.

In: Cancer Science, Vol. 96, No. 1, 01.2005, p. 26-30.

Research output: Contribution to journalArticle

Iwamori, Masao ; Tanaka, Kyoko ; Kubushiro, Kaneyuki ; Lin, Bei ; Kiguchi, Kazushige ; Ishiwata, Isamu ; Tsukazaki, Katsumi ; Nozawa, Shiro. / Alterations in the glycolipid composition and cellular properties of ovarian carcinoma-derived RMG-1 cells on transfection of the α1,2-fucosyltransferase gene. In: Cancer Science. 2005 ; Vol. 96, No. 1. pp. 26-30.
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