Alterations of the ocular surface epithelial mucins 1, 2, 4 and the tear functions in patients with atopic keratoconjunctivitis

M. Dogru, N. Okada, N. Asano-Kato, A. Igarashi, K. Fukagawa, J. Shimazaki, Kazuo Tsubota, H. Fujishima

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: An increased understanding of the ocular surface alterations at the cellular level in the conjunctiva and the cornea, may help explain the pathogenesis and the subsequent clinical appearance of atopic ocular allergies, which may be potentially blinding. Purpose: To investigate MUC 1, 2 and 4 alterations, tear function and the ocular surface disorder in patients with atopic keratoconjunctivitis. Methods: Twenty-eight eyes of 14 atopic keratoconjunctivitis patients as well as 22 eyes of 11 age-and sex-matched normal subjects were studied. The subjects underwent corneal sensitivity measurements, Schirmer's test, tear film break-up time (BUT), fluorescein and Rose Bengal staining of the ocular surface, conjunctival impression cytology and brush cytology. Impression cytology samples underwent periodic acid-Schiff and immunohistochemical staining with MUC 1, 2 and 4 antibodies. Brush cytology specimens underwent evaluation for inflammatory cell numbers and quantitative real-time-PCR for MUC 1, 2 and 4 mRNA expression. Patient eyes with fluorescein and Rose Bengal scores greater than four points were regarded to have significant epithelial disease in this study. Results: The mean corneal sensitivity and BUT values were significantly lower in atopic patients with significant epithelial disease, compared with patients with insignificant epithelial disease and controls (P<0.01). Brush cytology specimens from patients with significant epithelial disease revealed significantly higher numbers of inflammatory cells (P<0.01). Specimens from patient eyes showed positive staining for MUC 1, 2 and 4. MUC 1, 2 and 4 mRNA expressions were significantly higher in eyes with significant epithelial disease compared with eyes with insignificant epithelial disease and eyes of control subjects. Conclusion: Ocular surface inflammation, decline in corneal sensitivity, tear film instability, changes in conjunctival epithelial MUC 1, 2 and 4 mRNA expressions were thought to be important in the pathogenesis of atopic ocular surface disease.

Original languageEnglish
Pages (from-to)1556-1565
Number of pages10
JournalClinical and Experimental Allergy
Volume36
Issue number12
DOIs
Publication statusPublished - 2006 Dec

Fingerprint

Mucin-2
Keratoconjunctivitis
Mucin-1
Tears
Cell Biology
Rose Bengal
Eye Diseases
Staining and Labeling
Fluorescein
Messenger RNA
Cell Count
Periodic Acid
Conjunctiva
Cornea

Keywords

  • Atopy
  • Brush cytology
  • Conjunctival squamous metaplasia
  • Corneal sensitivity
  • Goblet cell
  • Impression cytology
  • Tear film

ASJC Scopus subject areas

  • Immunology

Cite this

Alterations of the ocular surface epithelial mucins 1, 2, 4 and the tear functions in patients with atopic keratoconjunctivitis. / Dogru, M.; Okada, N.; Asano-Kato, N.; Igarashi, A.; Fukagawa, K.; Shimazaki, J.; Tsubota, Kazuo; Fujishima, H.

In: Clinical and Experimental Allergy, Vol. 36, No. 12, 12.2006, p. 1556-1565.

Research output: Contribution to journalArticle

Dogru, M. ; Okada, N. ; Asano-Kato, N. ; Igarashi, A. ; Fukagawa, K. ; Shimazaki, J. ; Tsubota, Kazuo ; Fujishima, H. / Alterations of the ocular surface epithelial mucins 1, 2, 4 and the tear functions in patients with atopic keratoconjunctivitis. In: Clinical and Experimental Allergy. 2006 ; Vol. 36, No. 12. pp. 1556-1565.
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AU - Dogru, M.

AU - Okada, N.

AU - Asano-Kato, N.

AU - Igarashi, A.

AU - Fukagawa, K.

AU - Shimazaki, J.

AU - Tsubota, Kazuo

AU - Fujishima, H.

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KW - Brush cytology

KW - Conjunctival squamous metaplasia

KW - Corneal sensitivity

KW - Goblet cell

KW - Impression cytology

KW - Tear film

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