Altered expression of basement membrane-related molecules in rat brain pericyte, endothelial, and astrocyte cell lines after transforming growth factor-beta1 treatment.

Noriko Kose, Tomoko Asashima, Mariko Muta, Hisashi Iizasa, Yoshimichi Sai, Tetsuya Terasaki, Emi Nakashima

Research output: Contribution to journalArticle

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Abstract

The basement membrane at the blood-brain barrier (BBB) plays important roles in maintaining the structure and function of capillary vessels. The BBB is constructed from endothelial cells, astrocytes and pericytes, but their interactions in the formation or maintenance of basement membrane have not been established. Transforming growth factor-beta1 (TGF-beta1) is known to increase fibronectin in brain capillary basement membrane with deposition of beta-amyloid. We previously reported that the mRNA level of alpha-smooth muscle actin in a brain capillary pericyte cell line TR-PCT1 was increased by treatment with TGF-beta1. In this study, expression of mRNAs encoding basement membrane-related molecules in TR-PCT1, a rat endothelial cell line TR-BBB13, and a type 2 astrocyte cell line TR-AST4 was evaluated by RT-PCR. The effects of TGF-beta1 on expression of basement membrane-related genes in these cell lines were also examined. Fibronectin, MMP-9, tPA, TIMP-1, and PAI-l in TR-PCT1 were higher than in TR-BBB13 and TR-AST4. In TR-PCT1 treated with TGF-beta1, collagen type IV, PAI-1, and MMP-9 were increased, and TIMP-2 was reduced. The change in PAI-1 mRNA was faster than those in MMP-9, TIMP-2, collagen type IV mRNAs. These results suggest that pericytes may be key cells in the maintenance of the basement membrane at the BBB.

Original languageEnglish
Pages (from-to)255-266
Number of pages12
JournalDrug Metabolism and Pharmacokinetics
Volume22
Issue number4
DOIs
Publication statusPublished - 2007 Aug

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Transforming Growth Factor beta1
Pericytes
Basement Membrane
Astrocytes
Endothelial Cells
Cell Line
Brain
Blood-Brain Barrier
Matrix Metalloproteinases
Tissue Inhibitor of Metalloproteinase-2
Messenger RNA
Collagen Type IV
Plasminogen Activator Inhibitor 1
Fibronectins
Maintenance
Tissue Inhibitor of Metalloproteinase-1
Amyloid
Smooth Muscle
Actins
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

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Altered expression of basement membrane-related molecules in rat brain pericyte, endothelial, and astrocyte cell lines after transforming growth factor-beta1 treatment. / Kose, Noriko; Asashima, Tomoko; Muta, Mariko; Iizasa, Hisashi; Sai, Yoshimichi; Terasaki, Tetsuya; Nakashima, Emi.

In: Drug Metabolism and Pharmacokinetics, Vol. 22, No. 4, 08.2007, p. 255-266.

Research output: Contribution to journalArticle

Kose, Noriko ; Asashima, Tomoko ; Muta, Mariko ; Iizasa, Hisashi ; Sai, Yoshimichi ; Terasaki, Tetsuya ; Nakashima, Emi. / Altered expression of basement membrane-related molecules in rat brain pericyte, endothelial, and astrocyte cell lines after transforming growth factor-beta1 treatment. In: Drug Metabolism and Pharmacokinetics. 2007 ; Vol. 22, No. 4. pp. 255-266.
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