Altered expression of microRNA-223 in the plasma of patients with first-episode schizophrenia and its possible relation to neuronal migration-related genes

Zhilei Zhao, Seiichiro Jinde, Shinsuke Koike, Mariko Tada, Yoshihiro Satomura, Akane Yoshikawa, Yukika Nishimura, Ryu Takizawa, Akihide Kinoshita, Eisuke Sakakibara, Hanako Sakurada, Mika Yamagishi, Fumichika Nishimura, Aya Inai, Masaki Nishioka, Yosuke Eriguchi, Tsuyoshi Araki, Atsuhiko Takaya, Chiemi Kan, Maki UmedaAkihito Shimazu, Hideki Hashimoto, Miki Bundo, Kazuya Iwamoto, Chihiro Kakiuchi, Kiyoto Kasai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent studies have shown that microRNAs (miRNAs) play a role as regulators of neurodevelopment by modulating gene expression. Altered miRNA expression has been reported in various psychiatric disorders, including schizophrenia. However, the changes in the miRNA expression profile that occur during the initial stage of schizophrenia have not been fully investigated. To explore the global alterations in miRNA expression profiles that may be associated with the onset of schizophrenia, we first profiled miRNA expression in plasma from 17 patients with first-episode schizophrenia and 17 healthy controls using microarray analysis. Among the miRNAs that showed robust changes, the elevated expression of has-miR-223-3p (miR-223) was validated via quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using another independent sample set of 21 schizophrenia patients and 21 controls. To identify the putative targets of miR-223, we conducted a genome-wide gene expression analysis in neuronally differentiated SK-N-SH cells with stable miR-223 overexpression and an in silico analysis. We found that the mRNA expression levels of four genes related to the cytoskeleton or cell migration were significantly downregulated in miR-223-overexpressing cells, possibly due to interactions with miR-223. The in silico analysis suggested the presence of miR-223 target sites in these four genes. Lastly, a luciferase assay confirmed that miR-223 directly interacted with the 3′ untranslated regions (UTRs) of all four genes. Our results reveal an increase in miR-223 in plasma during both the first episode and the later stage of schizophrenia, which may affect the expression of cell migration-related genes targeted by miR-223.

Original languageEnglish
Article number289
JournalTranslational psychiatry
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience
  • Biological Psychiatry

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    Zhao, Z., Jinde, S., Koike, S., Tada, M., Satomura, Y., Yoshikawa, A., Nishimura, Y., Takizawa, R., Kinoshita, A., Sakakibara, E., Sakurada, H., Yamagishi, M., Nishimura, F., Inai, A., Nishioka, M., Eriguchi, Y., Araki, T., Takaya, A., Kan, C., ... Kasai, K. (2019). Altered expression of microRNA-223 in the plasma of patients with first-episode schizophrenia and its possible relation to neuronal migration-related genes. Translational psychiatry, 9(1), [289]. https://doi.org/10.1038/s41398-019-0609-0