Altered gene expression of uncoupling protein-2 and -3 in stroke-prone spontaneously hypertensive rats

Yasutomo Fukunaga, Hiroshi Itoh, Kiminori Hosoda, Kentaro Doi, Junichi Matsuda, Cheol Son, Jun Yamashita, Tae Hwa Chun, Tokuji Tanaka, Mayumi Inoue, Ken Masatsugu, Takatoshi Saito, Naoki Sawada, Kazuwa Nakao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Uncoupling proteins (UCPs) are the inner mitochondrial membrane-associated proteins, which dissipate the proton gradient and generate heat instead of ATP. The involvement of UCPs in energy expenditure and glucose metabolism has been suggested. Recently, we succeeded in cloning of rat UCP2 and UCP3. Objective: The aim of this study was to elucidate the pathophysiological role of UCP2 and UCP3 in hypertension associated with hyperglycemia in stroke-prone spontaneously hypertensive rats (SHR-SP). Methods: UCP2 and UCP3 mRNA levels of cardiac and gastrocnemius muscles in SHR-SP and Wistar-Kyoto (WKY) rats were determined at 6 weeks (prehypertensive stage) and at 15 weeks (hypertensive stage). Results: UCP2 and UCP3 mRNA levels in the heart of SHR-SP at 6 weeks were significantly higher than those of WKY rats (1.6-fold, 3.6-fold, respectively). These tendencies did not change in the heart at 15 weeks. UCP2 and UCP3 mRNA levels in the skeletal muscle of SHR-SP at 6 weeks were significantly higher than those of WKY rats (1.4-fold, 2.4-fold, respectively). In contrast, at 15 weeks, UCP2 and UCP3 mRNA levels in the skeletal muscle of SHR-SP were significantly lower than those of WKY rats (70 and 36% of WKY rats, respectively). Therefore, the decrease of UCP2 and UCP3 in the skeletal muscle was observed with the concomitant development of hypertension in SHR-SP. UCP2 mRNA levels in the epididymal fat of SHR-SP at 15 weeks were similar to that of WKY rats. Conclusions: Altered gene expression of UCP2 and UCP3 might be related to some pathophysiological aspects in hypertension and glucose metabolism in SHR-SP. (C) Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)1233-1238
Number of pages6
JournalJournal of Hypertension
Volume18
Issue number9
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Inbred WKY Rats
Inbred SHR Rats
Stroke
Gene Expression
Skeletal Muscle
Messenger RNA
Hypertension
Glucose
Mitochondrial Proteins
Mitochondrial Membranes
Hyperglycemia
Energy Metabolism
Uncoupling Protein 3
Uncoupling Protein 2
Protons
Organism Cloning
Myocardium
Membrane Proteins
Hot Temperature
Adenosine Triphosphate

Keywords

  • Heart
  • Hyperglycemia
  • Hypertension
  • Mitochondria
  • Skeletal muscle
  • Stroke-prone spontaneously hypertensive rat
  • Uncoupling protein

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology

Cite this

Fukunaga, Y., Itoh, H., Hosoda, K., Doi, K., Matsuda, J., Son, C., ... Nakao, K. (2000). Altered gene expression of uncoupling protein-2 and -3 in stroke-prone spontaneously hypertensive rats. Journal of Hypertension, 18(9), 1233-1238.

Altered gene expression of uncoupling protein-2 and -3 in stroke-prone spontaneously hypertensive rats. / Fukunaga, Yasutomo; Itoh, Hiroshi; Hosoda, Kiminori; Doi, Kentaro; Matsuda, Junichi; Son, Cheol; Yamashita, Jun; Chun, Tae Hwa; Tanaka, Tokuji; Inoue, Mayumi; Masatsugu, Ken; Saito, Takatoshi; Sawada, Naoki; Nakao, Kazuwa.

In: Journal of Hypertension, Vol. 18, No. 9, 2000, p. 1233-1238.

Research output: Contribution to journalArticle

Fukunaga, Y, Itoh, H, Hosoda, K, Doi, K, Matsuda, J, Son, C, Yamashita, J, Chun, TH, Tanaka, T, Inoue, M, Masatsugu, K, Saito, T, Sawada, N & Nakao, K 2000, 'Altered gene expression of uncoupling protein-2 and -3 in stroke-prone spontaneously hypertensive rats', Journal of Hypertension, vol. 18, no. 9, pp. 1233-1238.
Fukunaga, Yasutomo ; Itoh, Hiroshi ; Hosoda, Kiminori ; Doi, Kentaro ; Matsuda, Junichi ; Son, Cheol ; Yamashita, Jun ; Chun, Tae Hwa ; Tanaka, Tokuji ; Inoue, Mayumi ; Masatsugu, Ken ; Saito, Takatoshi ; Sawada, Naoki ; Nakao, Kazuwa. / Altered gene expression of uncoupling protein-2 and -3 in stroke-prone spontaneously hypertensive rats. In: Journal of Hypertension. 2000 ; Vol. 18, No. 9. pp. 1233-1238.
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abstract = "Background: Uncoupling proteins (UCPs) are the inner mitochondrial membrane-associated proteins, which dissipate the proton gradient and generate heat instead of ATP. The involvement of UCPs in energy expenditure and glucose metabolism has been suggested. Recently, we succeeded in cloning of rat UCP2 and UCP3. Objective: The aim of this study was to elucidate the pathophysiological role of UCP2 and UCP3 in hypertension associated with hyperglycemia in stroke-prone spontaneously hypertensive rats (SHR-SP). Methods: UCP2 and UCP3 mRNA levels of cardiac and gastrocnemius muscles in SHR-SP and Wistar-Kyoto (WKY) rats were determined at 6 weeks (prehypertensive stage) and at 15 weeks (hypertensive stage). Results: UCP2 and UCP3 mRNA levels in the heart of SHR-SP at 6 weeks were significantly higher than those of WKY rats (1.6-fold, 3.6-fold, respectively). These tendencies did not change in the heart at 15 weeks. UCP2 and UCP3 mRNA levels in the skeletal muscle of SHR-SP at 6 weeks were significantly higher than those of WKY rats (1.4-fold, 2.4-fold, respectively). In contrast, at 15 weeks, UCP2 and UCP3 mRNA levels in the skeletal muscle of SHR-SP were significantly lower than those of WKY rats (70 and 36{\%} of WKY rats, respectively). Therefore, the decrease of UCP2 and UCP3 in the skeletal muscle was observed with the concomitant development of hypertension in SHR-SP. UCP2 mRNA levels in the epididymal fat of SHR-SP at 15 weeks were similar to that of WKY rats. Conclusions: Altered gene expression of UCP2 and UCP3 might be related to some pathophysiological aspects in hypertension and glucose metabolism in SHR-SP. (C) Lippincott Williams and Wilkins.",
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author = "Yasutomo Fukunaga and Hiroshi Itoh and Kiminori Hosoda and Kentaro Doi and Junichi Matsuda and Cheol Son and Jun Yamashita and Chun, {Tae Hwa} and Tokuji Tanaka and Mayumi Inoue and Ken Masatsugu and Takatoshi Saito and Naoki Sawada and Kazuwa Nakao",
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T1 - Altered gene expression of uncoupling protein-2 and -3 in stroke-prone spontaneously hypertensive rats

AU - Fukunaga, Yasutomo

AU - Itoh, Hiroshi

AU - Hosoda, Kiminori

AU - Doi, Kentaro

AU - Matsuda, Junichi

AU - Son, Cheol

AU - Yamashita, Jun

AU - Chun, Tae Hwa

AU - Tanaka, Tokuji

AU - Inoue, Mayumi

AU - Masatsugu, Ken

AU - Saito, Takatoshi

AU - Sawada, Naoki

AU - Nakao, Kazuwa

PY - 2000

Y1 - 2000

N2 - Background: Uncoupling proteins (UCPs) are the inner mitochondrial membrane-associated proteins, which dissipate the proton gradient and generate heat instead of ATP. The involvement of UCPs in energy expenditure and glucose metabolism has been suggested. Recently, we succeeded in cloning of rat UCP2 and UCP3. Objective: The aim of this study was to elucidate the pathophysiological role of UCP2 and UCP3 in hypertension associated with hyperglycemia in stroke-prone spontaneously hypertensive rats (SHR-SP). Methods: UCP2 and UCP3 mRNA levels of cardiac and gastrocnemius muscles in SHR-SP and Wistar-Kyoto (WKY) rats were determined at 6 weeks (prehypertensive stage) and at 15 weeks (hypertensive stage). Results: UCP2 and UCP3 mRNA levels in the heart of SHR-SP at 6 weeks were significantly higher than those of WKY rats (1.6-fold, 3.6-fold, respectively). These tendencies did not change in the heart at 15 weeks. UCP2 and UCP3 mRNA levels in the skeletal muscle of SHR-SP at 6 weeks were significantly higher than those of WKY rats (1.4-fold, 2.4-fold, respectively). In contrast, at 15 weeks, UCP2 and UCP3 mRNA levels in the skeletal muscle of SHR-SP were significantly lower than those of WKY rats (70 and 36% of WKY rats, respectively). Therefore, the decrease of UCP2 and UCP3 in the skeletal muscle was observed with the concomitant development of hypertension in SHR-SP. UCP2 mRNA levels in the epididymal fat of SHR-SP at 15 weeks were similar to that of WKY rats. Conclusions: Altered gene expression of UCP2 and UCP3 might be related to some pathophysiological aspects in hypertension and glucose metabolism in SHR-SP. (C) Lippincott Williams and Wilkins.

AB - Background: Uncoupling proteins (UCPs) are the inner mitochondrial membrane-associated proteins, which dissipate the proton gradient and generate heat instead of ATP. The involvement of UCPs in energy expenditure and glucose metabolism has been suggested. Recently, we succeeded in cloning of rat UCP2 and UCP3. Objective: The aim of this study was to elucidate the pathophysiological role of UCP2 and UCP3 in hypertension associated with hyperglycemia in stroke-prone spontaneously hypertensive rats (SHR-SP). Methods: UCP2 and UCP3 mRNA levels of cardiac and gastrocnemius muscles in SHR-SP and Wistar-Kyoto (WKY) rats were determined at 6 weeks (prehypertensive stage) and at 15 weeks (hypertensive stage). Results: UCP2 and UCP3 mRNA levels in the heart of SHR-SP at 6 weeks were significantly higher than those of WKY rats (1.6-fold, 3.6-fold, respectively). These tendencies did not change in the heart at 15 weeks. UCP2 and UCP3 mRNA levels in the skeletal muscle of SHR-SP at 6 weeks were significantly higher than those of WKY rats (1.4-fold, 2.4-fold, respectively). In contrast, at 15 weeks, UCP2 and UCP3 mRNA levels in the skeletal muscle of SHR-SP were significantly lower than those of WKY rats (70 and 36% of WKY rats, respectively). Therefore, the decrease of UCP2 and UCP3 in the skeletal muscle was observed with the concomitant development of hypertension in SHR-SP. UCP2 mRNA levels in the epididymal fat of SHR-SP at 15 weeks were similar to that of WKY rats. Conclusions: Altered gene expression of UCP2 and UCP3 might be related to some pathophysiological aspects in hypertension and glucose metabolism in SHR-SP. (C) Lippincott Williams and Wilkins.

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KW - Hyperglycemia

KW - Hypertension

KW - Mitochondria

KW - Skeletal muscle

KW - Stroke-prone spontaneously hypertensive rat

KW - Uncoupling protein

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