Altered genie expression of natriuretic peptide receptor subtypes in the kidney of stroke-prone spontaneously hypertensive rats

M. Goto, Hiroshi Itoh, I. Tanaka, S. I. Suga, Y. Ogawa, I. Kishimoto, M. Nakagawa, A. Sugawara, T. Yoshimasa, M. Mukoyama, K. Nakao

Research output: Contribution to journalArticle

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Abstract

1. To elucidate the physiological and pathophysiological role of the natriuretic peptide system in the progression of hypertensive renal disease, we examined the gene expression of natriuretic peptide receptor subtypes, guanylate cyclase-A (GC-A), guanylate cyclase-B (GC-B) and clearance receptor (C receptor), in the kidney of stroke-prone spontaneously hypertensive rats (SHRSP) at 8 and 20 weeks of age, and compared them with their gene expression in age-matched Wistar-Kyoto (WKY) rats. 2. Northern blot analyses revealed that messages for three natriuretic peptide receptor subtypes were expressed in the kidney, and their expressions were higher in the glomeruli than in the whole kidney in each strain. 3. In 20 week old rats with established hypertension, the glomerular concentration of GC-A mRNA was significantly higher in SHRSP than in WKY. The concentrations of GC-B and C receptor mRNA in the glomeruli tended to increase and decrease, respectively, but they were not statistically significant in SHRSP. 4. In 8 week old rats, the glomerular concentrations of GC-A, GC-B and C receptor mRNA were not significantly different between SHRSP and WKY. 5. This study demonstrates that in the progression of hypertension, the expression of GC-A, which mediates biological actions of natriuretic peptides, is enhanced in the kidney of SHRSP compared to that of WKY. Together with the augmented secretion of the ligands previously revealed, altered expression of natriuretic peptide receptor subtypes in SHRSP may have a deterrent role in the development of hypertension and its renal complications.

Original languageEnglish
JournalClinical and Experimental Pharmacology and Physiology
Volume22
Issue numberSUPPL. 1
Publication statusPublished - 1995
Externally publishedYes

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Natriuretic Peptides
Peptide Receptors
Inbred SHR Rats
Guanylate Cyclase
Stroke
Kidney
Messenger RNA
Hypertension
Gene Expression
Renal Hypertension
Inbred WKY Rats
Northern Blotting
Genie
Ligands
atrial natriuretic factor receptor B

Keywords

  • Gene expression
  • Glomerulus
  • Guanylate cyclase
  • Hypertension
  • Kidney
  • Mesangial cells
  • Natriuretic peptides
  • receptor subtypes
  • Stroke-prone spontaneously hypertensive rat

ASJC Scopus subject areas

  • Physiology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Altered genie expression of natriuretic peptide receptor subtypes in the kidney of stroke-prone spontaneously hypertensive rats. / Goto, M.; Itoh, Hiroshi; Tanaka, I.; Suga, S. I.; Ogawa, Y.; Kishimoto, I.; Nakagawa, M.; Sugawara, A.; Yoshimasa, T.; Mukoyama, M.; Nakao, K.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 22, No. SUPPL. 1, 1995.

Research output: Contribution to journalArticle

Goto, M, Itoh, H, Tanaka, I, Suga, SI, Ogawa, Y, Kishimoto, I, Nakagawa, M, Sugawara, A, Yoshimasa, T, Mukoyama, M & Nakao, K 1995, 'Altered genie expression of natriuretic peptide receptor subtypes in the kidney of stroke-prone spontaneously hypertensive rats', Clinical and Experimental Pharmacology and Physiology, vol. 22, no. SUPPL. 1.
Goto, M. ; Itoh, Hiroshi ; Tanaka, I. ; Suga, S. I. ; Ogawa, Y. ; Kishimoto, I. ; Nakagawa, M. ; Sugawara, A. ; Yoshimasa, T. ; Mukoyama, M. ; Nakao, K. / Altered genie expression of natriuretic peptide receptor subtypes in the kidney of stroke-prone spontaneously hypertensive rats. In: Clinical and Experimental Pharmacology and Physiology. 1995 ; Vol. 22, No. SUPPL. 1.
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T1 - Altered genie expression of natriuretic peptide receptor subtypes in the kidney of stroke-prone spontaneously hypertensive rats

AU - Goto, M.

AU - Itoh, Hiroshi

AU - Tanaka, I.

AU - Suga, S. I.

AU - Ogawa, Y.

AU - Kishimoto, I.

AU - Nakagawa, M.

AU - Sugawara, A.

AU - Yoshimasa, T.

AU - Mukoyama, M.

AU - Nakao, K.

PY - 1995

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N2 - 1. To elucidate the physiological and pathophysiological role of the natriuretic peptide system in the progression of hypertensive renal disease, we examined the gene expression of natriuretic peptide receptor subtypes, guanylate cyclase-A (GC-A), guanylate cyclase-B (GC-B) and clearance receptor (C receptor), in the kidney of stroke-prone spontaneously hypertensive rats (SHRSP) at 8 and 20 weeks of age, and compared them with their gene expression in age-matched Wistar-Kyoto (WKY) rats. 2. Northern blot analyses revealed that messages for three natriuretic peptide receptor subtypes were expressed in the kidney, and their expressions were higher in the glomeruli than in the whole kidney in each strain. 3. In 20 week old rats with established hypertension, the glomerular concentration of GC-A mRNA was significantly higher in SHRSP than in WKY. The concentrations of GC-B and C receptor mRNA in the glomeruli tended to increase and decrease, respectively, but they were not statistically significant in SHRSP. 4. In 8 week old rats, the glomerular concentrations of GC-A, GC-B and C receptor mRNA were not significantly different between SHRSP and WKY. 5. This study demonstrates that in the progression of hypertension, the expression of GC-A, which mediates biological actions of natriuretic peptides, is enhanced in the kidney of SHRSP compared to that of WKY. Together with the augmented secretion of the ligands previously revealed, altered expression of natriuretic peptide receptor subtypes in SHRSP may have a deterrent role in the development of hypertension and its renal complications.

AB - 1. To elucidate the physiological and pathophysiological role of the natriuretic peptide system in the progression of hypertensive renal disease, we examined the gene expression of natriuretic peptide receptor subtypes, guanylate cyclase-A (GC-A), guanylate cyclase-B (GC-B) and clearance receptor (C receptor), in the kidney of stroke-prone spontaneously hypertensive rats (SHRSP) at 8 and 20 weeks of age, and compared them with their gene expression in age-matched Wistar-Kyoto (WKY) rats. 2. Northern blot analyses revealed that messages for three natriuretic peptide receptor subtypes were expressed in the kidney, and their expressions were higher in the glomeruli than in the whole kidney in each strain. 3. In 20 week old rats with established hypertension, the glomerular concentration of GC-A mRNA was significantly higher in SHRSP than in WKY. The concentrations of GC-B and C receptor mRNA in the glomeruli tended to increase and decrease, respectively, but they were not statistically significant in SHRSP. 4. In 8 week old rats, the glomerular concentrations of GC-A, GC-B and C receptor mRNA were not significantly different between SHRSP and WKY. 5. This study demonstrates that in the progression of hypertension, the expression of GC-A, which mediates biological actions of natriuretic peptides, is enhanced in the kidney of SHRSP compared to that of WKY. Together with the augmented secretion of the ligands previously revealed, altered expression of natriuretic peptide receptor subtypes in SHRSP may have a deterrent role in the development of hypertension and its renal complications.

KW - Gene expression

KW - Glomerulus

KW - Guanylate cyclase

KW - Hypertension

KW - Kidney

KW - Mesangial cells

KW - Natriuretic peptides

KW - receptor subtypes

KW - Stroke-prone spontaneously hypertensive rat

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