Alternative splicing of neurofibromatosis type 1 gene transcript in malignant brain tumors: PCR analysis of frozen-section mRNA

H. Mochizuki, T. Nishi, J. M. Bruner, P. S Y Lee, V. A. Levin, Hideyuki Saya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The neurofibromatosis type 1 (NF1) gene encodes a 360-residue region showing significant homology to the catalytic domains of both mammalian GTPase-activating protein (GAP) and yeast IRA protein. The product of the GAP-related domain of the NF1 gene (NF1-GRD) has been shown to stimulate ras GTPase and consequently to inactivate ras protein. We previously reported that the NF1-GRD has two types of transcripts, type I and type II, which are generated by an alternative splicing mechanism, and that the differential splicing of the NF1-GRD may be related to differentiation of neuroectodermal cells. Here we examined the differential expression of type I and type II transcripts of NF1-GRD in clinical samples of supratentorial malignant brain tumors by the RNA-polymerase chain reaction (PCR) method using frozen tissue sections. Our observations revealed that normal cerebrum predominantly expressed the type II NF1-GRD transcript, whereas primitive neuroectodermal tumors predominantly expressed the type I transcript. Additionally, although the type I/type II ratio in astrocytomas varied widely among tissue samples, all glioblastomas showed higher type I/type II ratios that adjacent brain samples. The RNA-PCR analysis using frozen tissue sections is a useful and sensitive method for detecting genetic markers in clinical tissue samples.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalMolecular Carcinogenesis
Volume6
Issue number2
DOIs
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Neurofibromatosis 1 Genes
Neurofibromatosis 1
Alternative Splicing
Frozen Sections
Brain Neoplasms
Polymerase Chain Reaction
Messenger RNA
Neurofibromatosis 2
GTPase-Activating Proteins
ras Proteins
DNA-Directed RNA Polymerases
Primitive Neuroectodermal Tumors
Fungal Proteins
Astrocytoma
Cerebrum
Glioblastoma
Genetic Markers
Cell Differentiation
Catalytic Domain

Keywords

  • Alternative splicing
  • Brain tumor
  • Frozen section RNA
  • Neurofibromatosis type 1 (NF1) gene
  • RNA-PCR

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Alternative splicing of neurofibromatosis type 1 gene transcript in malignant brain tumors : PCR analysis of frozen-section mRNA. / Mochizuki, H.; Nishi, T.; Bruner, J. M.; Lee, P. S Y; Levin, V. A.; Saya, Hideyuki.

In: Molecular Carcinogenesis, Vol. 6, No. 2, 1992, p. 83-87.

Research output: Contribution to journalArticle

Mochizuki, H. ; Nishi, T. ; Bruner, J. M. ; Lee, P. S Y ; Levin, V. A. ; Saya, Hideyuki. / Alternative splicing of neurofibromatosis type 1 gene transcript in malignant brain tumors : PCR analysis of frozen-section mRNA. In: Molecular Carcinogenesis. 1992 ; Vol. 6, No. 2. pp. 83-87.
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