Aluminum induces tau aggregation in vitro but not in vivo

Tatsuya Mizoroki, Shunsuke Meshitsuka, Sumihiro Maeda, Miyuki Murayama, Naruhiko Sahara, Akihiko Takashima

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Etiological studies suggest that aluminum (Al) intake might increase an individual's risk of developing Alzheimer's disease (AD). Biochemical analysis data on the effects of Al, however, are inconsistent. Hence, the pathological involvement of Al in AD remains unclear. If Al is involved in AD, then it is reasonable to hypothesize that Al might be involved in the formation of either amyloid plaques or neurofibrillary tangles (NFTs). Here, we investigated whether Al might be involved in NFT formation by using an in vitro tau aggregation paradigm, a tau-overexpressing neuronal cell line (N2a), and a tau-overexpressing mouse model. Although Al induced tau aggregation in a heparin-induced tau assembly assay, these aggregates were neither thioflavin T positive nor did they resemble tau fibrils seen in human AD brains. With cell lysates from stable cell lines overexpressing tau, the accumulation of SDS-insoluble tau increased when the lysates were treated with at least 100 μM Al-maltolate. Yet Al-maltolate caused illness or death in transgenic mice overexpressing human tau and in non-transgenic littermates well before the Al concentration in the brain reached 100 μM. These results indicate that Al has no direct link to AD pathology.

Original languageEnglish
Pages (from-to)419-427
Number of pages9
JournalJournal of Alzheimer's Disease
Volume11
Issue number4
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Aluminum
Alzheimer Disease
Neurofibrillary Tangles
In Vitro Techniques
Cell Line
Amyloid Plaques
Brain
Transgenic Mice
Heparin
Pathology

Keywords

  • Aluminum
  • Alzheimer's disease
  • Tau aggregation
  • Tau fibril formation
  • Tau transgenic mouse

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology

Cite this

Mizoroki, T., Meshitsuka, S., Maeda, S., Murayama, M., Sahara, N., & Takashima, A. (2007). Aluminum induces tau aggregation in vitro but not in vivo. Journal of Alzheimer's Disease, 11(4), 419-427.

Aluminum induces tau aggregation in vitro but not in vivo. / Mizoroki, Tatsuya; Meshitsuka, Shunsuke; Maeda, Sumihiro; Murayama, Miyuki; Sahara, Naruhiko; Takashima, Akihiko.

In: Journal of Alzheimer's Disease, Vol. 11, No. 4, 2007, p. 419-427.

Research output: Contribution to journalArticle

Mizoroki, T, Meshitsuka, S, Maeda, S, Murayama, M, Sahara, N & Takashima, A 2007, 'Aluminum induces tau aggregation in vitro but not in vivo', Journal of Alzheimer's Disease, vol. 11, no. 4, pp. 419-427.
Mizoroki T, Meshitsuka S, Maeda S, Murayama M, Sahara N, Takashima A. Aluminum induces tau aggregation in vitro but not in vivo. Journal of Alzheimer's Disease. 2007;11(4):419-427.
Mizoroki, Tatsuya ; Meshitsuka, Shunsuke ; Maeda, Sumihiro ; Murayama, Miyuki ; Sahara, Naruhiko ; Takashima, Akihiko. / Aluminum induces tau aggregation in vitro but not in vivo. In: Journal of Alzheimer's Disease. 2007 ; Vol. 11, No. 4. pp. 419-427.
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